FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7921
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC173721.929049362454236No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC153119.328367630349703No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC128216.184825148339858No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG81110.238606236586289No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC6988.812018684509532No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG1832.310314354248201No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1672.1083196566090137No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG490.6186087615200101No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.39136472667592476No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT300.37874005807347555No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA280.3534907208685772No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.3156167150612297No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT190.23986870344653452No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.22724403484408537No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.21461936624163616No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT170.21461936624163616No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT160.20199469763918695No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT160.20199469763918695No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.20199469763918695No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT160.20199469763918695No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.18937002903673777No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.1767453604342886No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.1767453604342886No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.1767453604342886No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.16412069183183942No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.16412069183183942No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.16412069183183942No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT120.15149602322939024No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.15149602322939024No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT120.15149602322939024No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.15149602322939024No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.12624668602449185No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA100.12624668602449185No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.11362201742204268No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.11362201742204268No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT90.11362201742204268No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.11362201742204268No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAAAGACGTGTAGAA90.11362201742204268No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT80.10099734881959348No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.10099734881959348No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT80.10099734881959348No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC80.10099734881959348No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)