FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008150993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008150993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7609
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG509666.97332106715731No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC90911.946379287685636No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT3124.100407412274937No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC3064.021553423577343No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC520.6834012353791562No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC350.45998160073597055No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG280.36798528058877644No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT230.3022736233407806No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTCGTGAGACCAAGTCTCT220.2891312918911815No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG180.23656196609278488No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC170.2234196346431857No Hit
TGTGTGTGTGTTTTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC160.21027730319358653No Hit
TGTTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC130.17085030884478905No Hit
TTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC120.1577079773951899No Hit
TGTGTGTGTGTTTCTTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC120.1577079773951899No Hit
TGTGTGGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC120.1577079773951899No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG110.14456564594559074No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTCGTGAGACCAAGTCTCT110.14456564594559074No Hit
TGTGTGTGTGTTTCTGTGAGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG100.1314233144959916No Hit
TGTGTGTGTGTTTCGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC100.1314233144959916No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAGAG90.11828098304639244No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC90.11828098304639244No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTGAAG90.11828098304639244No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT90.11828098304639244No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT90.11828098304639244No Hit
TGTGTGTGGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC90.11828098304639244No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGGAGGGTAAAG80.10513865159679327No Hit
TGTGGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC80.10513865159679327No Hit
TGTGTTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC80.10513865159679327No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACGGAAGGGTAAAG80.10513865159679327No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG80.10513865159679327No Hit
TGGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC80.10513865159679327No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)