FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4391
Total Bases636.6 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG132430.152584832612163No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT117126.66818492370758No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC83418.99339558187201No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC2716.1717148713277155No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG1403.1883397859257574No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC370.8426326577089501No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC300.6832156684126622No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTCGTGAGACCAAGTCTCT230.5237986791163743No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT190.43270325666135273No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG150.3416078342063311No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC130.2960601229788203No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTCGTGAGACCAAGTCTCT110.2505124117513095No Hit
ACTTAAAAACAAGGCAGCTCTGAAACGGTATTTTTCGTGAGACCAAGTCT110.2505124117513095No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTCGTGAGACCAAGTCTCT90.20496470052379867No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC80.18219084491004328No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC70.15941698929628786No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT70.15941698929628786No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT70.15941698929628786No Hit
ACTTAAAAACAAGGCAGTGCTGCCGGAGCAAAAGCGGTAGCGGGAGCCCG60.13664313368253245No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC60.13664313368253245No Hit
ACTTAAAAACAAGGCAGCTCTGAAGGTATTTTTCGTGAGACCAAGTCTCT60.13664313368253245No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG50.11386927806877704No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCCAATGGTGCTACAGTTTCTGCCT50.11386927806877704No Hit
ACTTAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAGC50.11386927806877704No Hit
TGTGTGTGTGTTTCGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC50.11386927806877704No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC50.11386927806877704No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTCGTGAGACCAAGTCTCT50.11386927806877704No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)