FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9528
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC419844.05961376994122No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC115712.143157010915196No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG108711.40848026868178No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC6106.402183039462636No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG3353.515952980688497No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC1601.6792611251049538No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1371.4378673383711167No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG710.7451721242653232No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA630.6612090680100755No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA490.5142737195633921No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA370.3883291351805206No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA370.3883291351805206No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA350.36733837111670864No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA330.34634760705289674No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.3253568429890848No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.3148614609571789No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA280.2938706968933669No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT270.28337531486146095No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.2518891687657431No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT240.2518891687657431No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT210.2204030226700252No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC200.20990764063811923No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA190.19941225860621326No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC180.1889168765743073No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT170.17842149454240136No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.16792611251049538No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT140.14693534844668346No Hit
GGCACTAGAATGGACCTCTTCTCATCACTACATTATTTCAATTAAATACA140.14693534844668346No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC130.1364399664147775No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.12594458438287154No Hit
AAATACCGTTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAAC120.12594458438287154No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC120.12594458438287154No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA100.10495382031905962No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAACAC100.10495382031905962No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT100.10495382031905962No Hit
GGCACTAGAATGGACCTCTTACTCATCACTACATTATTTCAATTAAATAC100.10495382031905962No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.10495382031905962No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT100.10495382031905962No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.10495382031905962No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.10495382031905962No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAAAGACGTGTAGAA100.10495382031905962No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)