FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7242
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG423558.47832090582712No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT92312.745098039215685No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC5597.718862192764429No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC3064.225352112676056No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG1962.7064346865506765No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC1532.112676056338028No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC410.5661419497376415No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT340.4694835680751174No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC280.38663352665009665No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC250.3452085059375863No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG230.31759182546257936No Hit
TGTGTGTGTGTTTTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC160.22093344380005522No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG150.2071251035625518No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC150.2071251035625518No Hit
TGTGTGGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC140.19331676332504832No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTCGTGAGACCAAGTCTCT140.19331676332504832No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT120.16570008285004142No Hit
ACTTAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT110.151891742612538No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGGAGGGTAAAG110.151891742612538No Hit
TGTGTGTTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC100.13808340237503453No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACGGAAGGGTAAAG100.13808340237503453No Hit
TGGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC100.13808340237503453No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTGAAG80.11046672190002761No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC80.11046672190002761No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTCTAAGGTTTGGACAGAAGGGTAAAG80.11046672190002761No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC80.11046672190002761No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG80.11046672190002761No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAGGGTTTGGACAGAAGGGTAAAG80.11046672190002761No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)