FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9407
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC329635.037737854788986No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC208722.18560646327203No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC7167.611353247581588No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG6697.111725310938663No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC6496.899117678324653No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT2142.2749016689699157No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG1391.4776230466673752No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA470.49962793664292554No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG420.4464760284894228No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT330.3508025938131179No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.34017221218241733No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.30828106729031574No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA290.30828106729031574No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA280.29765068565961517No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.2763899223982141No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA260.2763899223982141No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.2232380142447114No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.21260763261401086No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.2019772509833103No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.19134686935260978No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT170.1807164877219092No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.15945572446050812No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.15945572446050812No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT150.15945572446050812No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.15945572446050812No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.15945572446050812No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.14882534282980758No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.14882534282980758No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.11693419793770597No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.11693419793770597No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC110.11693419793770597No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.11693419793770597No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.10630381630700543No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC100.10630381630700543No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)