FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4149
Total Bases601.4 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC117928.41648590021692No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC59514.340805013256205No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC52112.557242709086527No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG51812.484936129187755No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC2896.9655338635815855No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG1513.63943118823813No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT882.1209930103639434No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA200.4820438659918052No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG200.4820438659918052No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT190.45794167269221503No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.43383947939262474No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.43383947939262474No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT180.43383947939262474No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT150.3615328994938539No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.28922631959508316No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA120.28922631959508316No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA110.2651241262954929No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.2410219329959026No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT100.2410219329959026No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.21691973969631237No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT90.21691973969631237No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.14461315979754158No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.14461315979754158No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.14461315979754158No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.14461315979754158No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.14461315979754158No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC60.14461315979754158No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.14461315979754158No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.14461315979754158No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC60.14461315979754158No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.14461315979754158No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.1205109664979513No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTAATTCAATTAAATAC50.1205109664979513No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.1205109664979513No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA50.1205109664979513No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT50.1205109664979513No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT50.1205109664979513No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT50.1205109664979513No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)