FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9340
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC297031.798715203426127No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC212022.698072805139187No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG8168.736616702355459No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC7788.329764453961456No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC7157.655246252676659No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG2292.4518201284796577No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG660.7066381156316917No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA530.5674518201284797No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.417558886509636No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA330.3533190578158458No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.2890792291220557No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.2890792291220557No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.26766595289079226No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT230.2462526766595289No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.23554603854389722No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT220.23554603854389722No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.23554603854389722No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.22483940042826553No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA210.22483940042826553No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT190.20342612419700215No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.20342612419700215No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.20342612419700215No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.20342612419700215No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.20342612419700215No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.19271948608137046No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.19271948608137046No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTAAATTATTTCAATTAAATAC180.19271948608137046No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT170.18201284796573877No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA160.17130620985010706No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.17130620985010706No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.16059957173447537No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.14989293361884368No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.14989293361884368No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC130.139186295503212No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.11777301927194861No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT110.11777301927194861No Hit
GGCACTAGAATGGACCTCTTCTCATCACTAAATTATTTCAATTAAATACA110.11777301927194861No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC100.10706638115631692No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC100.10706638115631692No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)