FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3220
Total Bases466.7 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC108533.69565217391305No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC49415.341614906832298No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC45314.06832298136646No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG2557.919254658385093No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC2016.24223602484472No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTATATTATTTCAATTAAATAC1173.633540372670808No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG471.4596273291925466No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.5279503105590062No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.37267080745341613No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG120.37267080745341613No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.3105590062111801No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.27950310559006214No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.2484472049689441No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC80.2484472049689441No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.2484472049689441No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT80.2484472049689441No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.21739130434782608No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.21739130434782608No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT70.21739130434782608No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.21739130434782608No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.21739130434782608No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.18633540372670807No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC60.18633540372670807No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA50.15527950310559005No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.15527950310559005No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.15527950310559005No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.15527950310559005No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA50.15527950310559005No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.12422360248447205No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.12422360248447205No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.12422360248447205No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.12422360248447205No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.12422360248447205No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.12422360248447205No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT40.12422360248447205No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)