FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6771
Total Bases981.4 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC279641.29375276916261No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC70810.456357997341604No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC5938.757938266134987No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG5257.753655294638902No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC4676.8970609954216515No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT2403.5445281346920687No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG791.166740511002806No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG470.6941367597105302No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA450.664599025254763No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA310.45783488406439227No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT310.45783488406439227No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA260.38399054792497417No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.35445281346920693No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.33968394624132325No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.3249150790134397No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.31014621178555607No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.31014621178555607No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.31014621178555607No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT180.2658396101019052No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT170.2510707428740216No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT150.2215330084182543No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.19199527396248708No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA120.17722640673460346No Hit
GGCACTAGAATGGACCTCTTCTCATCACTACATTATTTCAATTAAATACA120.17722640673460346No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGAAAGCCAATGGGAAA110.16245753950671984No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC110.16245753950671984No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC110.16245753950671984No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.1476886722788362No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATGC90.1329198050509526No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC90.1329198050509526No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT80.11815093782306897No Hit
GGCACTAGAATGGACCTTTTCCTCATCACTACATTATTTCAATTAAATAC80.11815093782306897No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC80.11815093782306897No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.11815093782306897No Hit
AATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACTG80.11815093782306897No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTACATTATTTCAATTAAATAC70.10338207059518535No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT70.10338207059518535No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTACATTATTTCAATTAAATAC70.10338207059518535No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT70.10338207059518535No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)