FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6486
Total Bases940 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC213732.947887758248534No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC146822.633364168979337No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC76311.763798951588036No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG3865.951279679309281No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC2714.178230033919211No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1702.6210299105766266No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG580.8942337341967314No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG350.5396238051187172No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA280.43169904409497384No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA270.4162812210915819No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.37002775208140615No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT230.3546099290780142No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.33919210607462225No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA210.32377428307123035No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.2929386370644465No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA190.2929386370644465No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.27752081406105455No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.21584952204748692No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.20043169904409497No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC130.20043169904409497No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.20043169904409497No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.18501387604070307No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA120.18501387604070307No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.18501387604070307No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.18501387604070307No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAAAGACGTGTAGAA120.18501387604070307No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT120.18501387604070307No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.16959605303731112No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.16959605303731112No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT110.16959605303731112No Hit
GGCACTAGAATGGACCTCTTCAGGCTTCCTTACTGCCCTATCCATCACCC110.16959605303731112No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA110.16959605303731112No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.1541782300339192No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.1541782300339192No Hit
AAATACCGTTCAGAGCTGCCATTGCCCCACCCACTTCTCTGGTAGAGAAA100.1541782300339192No Hit
GGCACTAGAATGGACCTCTTCTCATCACTAAATTATTTCAATTAAATACA100.1541782300339192No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC90.13876040703052728No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.13876040703052728No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.12334258402713535No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTAAATTATTTCAATTAAATAC80.12334258402713535No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTAAATTATTTCAATTAAATAC70.10792476102374346No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT70.10792476102374346No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT70.10792476102374346No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.10792476102374346No Hit
AAATACCGTTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAAC70.10792476102374346No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)