FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9894
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC421742.62179098443501No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC172917.475237517687486No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG9669.76349302607641No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC6526.589852435819689No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG2973.001819284414797No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC2302.3246411966848597No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA660.667070952092177No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG580.586213866990095No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA430.43460683242369114No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.3941782898726501No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA390.3941782898726501No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA370.3739640185971296No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.32342834040832824No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA300.3032140691328078No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT300.3032140691328078No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.2931069334950475No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA250.25267839094400646No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.2425712553062462No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT220.22235698403072568No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC210.21224984839296543No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA190.1920355771174449No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA170.1718213058419244No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA160.16171417020416412No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC150.1516070345664039No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.1516070345664039No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.1516070345664039No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.13139276329088337No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.13139276329088337No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT130.13139276329088337No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT130.13139276329088337No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.13139276329088337No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA130.13139276329088337No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.13139276329088337No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT120.1212856276531231No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.1212856276531231No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT120.1212856276531231No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT100.1010713563776026No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)