FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9410
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG598363.58129649309245No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT133314.165781083953242No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC5595.940488841657811No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG1982.104144527098831No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC1191.2646121147715197No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC500.5313496280552604No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG430.45696068012752394No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC380.4038257173219979No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC320.34006376195536664No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG300.3188097768331562No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT240.255047821466525No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC240.255047821466525No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGGAGGGTAAAG200.21253985122210414No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT200.21253985122210414No Hit
TGTGTGTGTGTTTTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC180.19128586609989373No Hit
TGTGTGTTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC170.1806588735387885No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG170.1806588735387885No Hit
TGTGTGGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC160.17003188097768332No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG160.17003188097768332No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAGGGTTTGGACAGAAGGGTAAAG160.17003188097768332No Hit
TGTTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC160.17003188097768332No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTGAAG150.1594048884165781No Hit
TGTGTTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC140.1487778958554729No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT130.13815090329436772No Hit
TTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC130.13815090329436772No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC130.13815090329436772No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAGAG120.1275239107332625No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC100.10626992561105207No Hit
TGTGTGTGTGTTTCTGTGGGTCTCTTTAAGGTTTGGACAGAAGGGTAAAG100.10626992561105207No Hit
TGTGTGTGTGTTTCGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC100.10626992561105207No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)