FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8388
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC292834.90701001430615No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC178621.292322365283738No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG157518.776824034334762No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC7619.07248450166905No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC1071.2756318550309966No Hit
ACTTAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT961.144492131616595No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT550.6556986170720076No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTCGTGAGACCAAGTCTCT450.5364806866952789No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT330.39341917024320455No Hit
ACTTAAAAACAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT230.2742012398664759No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCTT230.2742012398664759No Hit
ACTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT200.2384358607534573No Hit
ACTTAAAAAAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT190.22651406771578447No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTCGTGAGACCAAGTCTCT190.22651406771578447No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTCGTGAGACCAAGTCTCT180.2145922746781116No Hit
ACTTAAAAACAAGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT170.20267048164043872No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTCGTGAGACCAAGTCTCT150.17882689556509299No Hit
ACTTAAAAACAAGGAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT140.16690510252742013No Hit
ACTTAAAAACAAGGCAGTGTTAATGCTAATGGTGCTACAGTTTCTGCCTC130.15498330948974726No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC120.14306151645207438No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGTTACAGTTTCTGCCTC110.13113972341440153No Hit
ATTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT110.13113972341440153No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT110.13113972341440153No Hit
ACTTAAAAACAAGGCAGCTCTGAAACGGTATTTTTCGTGAGACCAAGTCT100.11921793037672865No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGTCTC90.1072961373390558No Hit
ACTTAAAAACAAGGCAGCTCTGAAGGTATTTTTCGTGAGACCAAGTCTCT90.1072961373390558No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTGCAGTTTCTGCCTC90.1072961373390558No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTCGTGAGACCAAGTCTCT90.1072961373390558No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)