FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12962
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC337426.029933652214165No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC237118.291930257676285No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG186914.419071130998304No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC11729.041814534794012No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG6545.045517667026694No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC5394.158308902947076No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT2812.1678753278814997No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG1301.0029316463508717No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA770.5940441289924394No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA590.4551766702669341No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT520.4011726585403487No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA500.3857429409041815No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT430.33173892917759606No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT410.3163092115414288No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA410.3163092115414288No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.27773491745101064No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT330.25459034099675976No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA300.23144576454250887No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA290.22373090572442525No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT280.2160160469063416No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA270.208301188088258No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA250.19287147045209074No Hit
AAATACCGTTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAAC230.17744175281592345No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.17744175281592345No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT210.1620120351797562No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT210.1620120351797562No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA190.14658231754358897No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC190.14658231754358897No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT190.14658231754358897No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.13886745872550532No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT170.1311525999074217No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT150.11572288227125443No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.11572288227125443No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.11572288227125443No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.11572288227125443No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT150.11572288227125443No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT140.1080080234531708No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT140.1080080234531708No Hit
AAATACCGTTCAGAGCGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT140.1080080234531708No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC130.10029316463508718No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT130.10029316463508718No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)