FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1680
Total Bases243.5 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC54032.142857142857146No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG42525.297619047619047No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC20412.142857142857142No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG1569.285714285714286No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC181.0714285714285714No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.7142857142857143No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT110.6547619047619048No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.5952380952380952No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.5952380952380952No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG100.5952380952380952No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.4761904761904762No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.4761904761904762No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT70.4166666666666667No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT60.35714285714285715No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT60.35714285714285715No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA60.35714285714285715No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT60.35714285714285715No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA50.2976190476190476No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.2380952380952381No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC40.2380952380952381No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.17857142857142858No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.17857142857142858No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.17857142857142858No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.17857142857142858No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.17857142857142858No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT30.17857142857142858No Hit
AAATACCGTTCAAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.17857142857142858No Hit
AAATACCTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.17857142857142858No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGCACCATG20.11904761904761905No Hit
GGCACTAGAATGGGCCTCTTCCTCATCACTACATTATTTCAATTAAATAC20.11904761904761905No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAAATAAATAC20.11904761904761905No Hit
AAATACCGTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.11904761904761905No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.11904761904761905No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACACTATTTCAATTAAATAC20.11904761904761905No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.11904761904761905No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC20.11904761904761905No Hit
AAATACAGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG20.11904761904761905No Hit
GGCACGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACAA20.11904761904761905No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAATAAGACGTGTAGAACCATGT20.11904761904761905No Hit
GGCACTAGAATGGATCTCTTCCTCATCACTACATTATTTCAATTAAATAC20.11904761904761905No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTAAATTAAATAC20.11904761904761905No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTAAATTAAATAC20.11904761904761905No Hit
GGCACTAGAATGGACCTCTCCTCATCACTACATTATTTCAATTAAATACA20.11904761904761905No Hit
AAATACCGTTCAGAGCTGCCATGTTTTTAAGTAAGACGTGTAGAACCATG20.11904761904761905No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC20.11904761904761905No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAAACATG20.11904761904761905No Hit
GGCACGAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC20.11904761904761905No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGC20.11904761904761905Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTAGAATGGACCTTTTCCTCATCACTACATTATTTCAATTAAATAC20.11904761904761905No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.11904761904761905No Hit
AAATACCGTTCAGAGCTGCCTTGCTTTTAAGTAAGACGTGTAGAACCATG20.11904761904761905No Hit
GGCACTAGAATGGACCTCTTCTCATCACTACATTATTTCAATTAAATACA20.11904761904761905No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCCTTGTTTTTAAGTAA20.11904761904761905Clontech SMARTer II A Oligonucleotide (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)