FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2764
Total Bases400.7 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC105138.0246020260492No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG66724.131693198263388No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG2629.479015918958032No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC1294.667149059334299No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC682.460202604920405No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG411.4833574529667148No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT401.447178002894356No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT230.8321273516642547No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT160.5788712011577424No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT160.5788712011577424No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT140.5065123010130246No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.361794500723589No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC80.2894356005788712No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.2532561505065123No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT70.2532561505065123No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT60.21707670043415342No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT60.21707670043415342No Hit
AAATACCGTTCAGACTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.21707670043415342No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC50.1808972503617945No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGGACC50.1808972503617945No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT50.1808972503617945No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTCCATGTACTCTGC50.1808972503617945Clontech Universal Primer Mix Long (95% over 23bp)
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.1808972503617945No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.1447178002894356No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA40.1447178002894356No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTACAAATTGCTGCTGGAACC40.1447178002894356No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.1447178002894356No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGC40.1447178002894356Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAGCC30.10853835021707671No Hit
AAATACCGTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.10853835021707671No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.10853835021707671No Hit
AAATACCGTTCAGAGCAGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC30.10853835021707671No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGTTGGAACC30.10853835021707671No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAGATTGCTGCTGGAACC30.10853835021707671No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAACTGCTGCTGGAACC30.10853835021707671No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.10853835021707671No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATAGCTGCTGGAACC30.10853835021707671No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.10853835021707671No Hit
AAATACCGTTCAGGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.10853835021707671No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.10853835021707671No Hit
AATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.10853835021707671No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAATAAGACGTGTAGAACCATGT30.10853835021707671No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTAGCAAATTGCTGCTGGAACC30.10853835021707671No Hit
AAATACCGTTCAAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.10853835021707671No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA30.10853835021707671No Hit
AAATACCGTTCAGAGCTGCCTTTTTTTAAGTAAGACGTGTAGAACCATGT30.10853835021707671No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT30.10853835021707671No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)