FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2827
Total Bases409.7 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC82029.006013441811106No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC43215.281216837637071No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC40514.326140785284755No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG37613.300318358684118No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG1314.633887513264945No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC832.9359745313052703No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG190.6720905553590378No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.5659709939865581No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT160.5659709939865581No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.4244782454899187No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.3537318712415989No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.3537318712415989No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT90.31835868411743895No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.2829854969932791No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT80.2829854969932791No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.2829854969932791No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT80.2829854969932791No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.24761230986911922No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA70.24761230986911922No Hit
AAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.21223912274495935No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.21223912274495935No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.21223912274495935No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.21223912274495935No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA50.17686593562079944No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT50.17686593562079944No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.17686593562079944No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.17686593562079944No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT50.17686593562079944No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.17686593562079944No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.14149274849663954No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.14149274849663954No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.14149274849663954No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT40.14149274849663954No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.10611956137247967No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.10611956137247967No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.10611956137247967No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA30.10611956137247967No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC30.10611956137247967No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.10611956137247967No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.10611956137247967No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.10611956137247967No Hit
GGCACTAGAATGGACCTCTTCCTCATCGCTACATTATTTCAATTAAATAC30.10611956137247967No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.10611956137247967No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAGTTAAATAC30.10611956137247967No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCCGGAACC30.10611956137247967No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT30.10611956137247967No Hit
AAATACGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.10611956137247967No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)