FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8636
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC359841.66280685502547No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC147817.114404817044928No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG122414.173228346456693No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG5346.183418249189439No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA810.9379342288096341No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG680.7874015748031495No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA490.5673923112552107No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA490.5673923112552107No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA490.5673923112552107No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA460.5326540064844836No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT440.5094951366373321No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT390.45159796201945346No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA370.42843909217230197No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA360.41685965724872626No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA350.40528022232515054No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT350.40528022232515054No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA320.37054191755442334No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA300.3473830477072719No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA190.22000926354793887No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA180.20842982862436313No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT150.17369152385363595No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.17369152385363595No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.15053265400648447No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT130.15053265400648447No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.15053265400648447No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC120.13895321908290875No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC120.13895321908290875No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT110.12737378415933304No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.12737378415933304No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.12737378415933304No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT110.12737378415933304No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC100.1157943492357573No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.1157943492357573No Hit
AAATACCGTTCGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT90.10421491431218156No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT90.10421491431218156No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTAAATTAAATAC90.10421491431218156No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)