FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5234
Total Bases758.6 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC157930.168131448223157No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG107020.443255636224684No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC90017.19526175009553No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG4418.42567825754681No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC1142.1780664883454337No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG390.7451280091708062No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA380.7260221627818112No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT280.5349636988918609No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA260.4967520061138708No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA240.45854031333588074No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT220.42032862055789066No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA220.42032862055789066No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.4012227741688957No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.4012227741688957No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT170.3247993886129156No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.3247993886129156No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT160.30569354222392053No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.2865876958349255No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT150.2865876958349255No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.2483760030569354No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA120.22927015666794037No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.19105846388995032No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT100.19105846388995032No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC100.19105846388995032No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA100.19105846388995032No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT100.19105846388995032No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.1719526175009553No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT90.1719526175009553No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.15284677111196027No Hit
AAATCCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.11463507833397019No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT60.11463507833397019No Hit
AAATACCGTTCGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT60.11463507833397019No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT60.11463507833397019No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC60.11463507833397019No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)