FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4509
Total Bases653.5 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC152133.73253493013972No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC88219.560878243512974No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG55512.308715901530274No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC3497.740075404746063No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG2365.233976491461521No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC400.8871146595697494No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA300.665335994677312No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG270.5988023952095809No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.4435573297848747No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.4435573297848747No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.4435573297848747No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT160.35484586382789973No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.332667997338656No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.3104901308494123No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.3104901308494123No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.2661343978709248No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT120.2661343978709248No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT110.2439565313816811No Hit
GGCACTAGAATGGACCTCTTCTCATCACTAAATTATTTCAATTAAATACA110.2439565313816811No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.22177866489243736No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT100.22177866489243736No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.22177866489243736No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.22177866489243736No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.19960079840319359No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.17742293191394987No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT80.17742293191394987No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA80.17742293191394987No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.17742293191394987No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT80.17742293191394987No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT80.17742293191394987No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTAAATTAAATAC80.17742293191394987No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.17742293191394987No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTAAATTATTTCAATTAAATAC70.15524506542470615No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT70.15524506542470615No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA70.15524506542470615No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT70.15524506542470615No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.1330671989354624No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC60.1330671989354624No Hit
AAATACCGTTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAAC60.1330671989354624No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.11088933244621868No Hit
GGCACTAGAATGGACCTCTTCCTCGTCACTAAATTATTTCAATTAAATAC50.11088933244621868No Hit
GGCACTAGAATGGACTTCTTCCTCATCACTAAATTATTTCAATTAAATAC50.11088933244621868No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC50.11088933244621868No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT50.11088933244621868No Hit
AGGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATA50.11088933244621868No Hit
GGCACTAGAATGGACCTCTTCTCATCACTACATTATTTCAATTAAATACA50.11088933244621868No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)