FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3403
Total Bases493.4 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG168149.39759036144578No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT72221.216573611519248No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC38111.196003526300323No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC1985.818395533352924No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC240.7052600646488393No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC190.5583308845136644No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTCGTGAGACCAAGTCTCT100.29385836027034967No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC90.2644725242433147No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT80.23508668821627976No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC70.2057008521892448No Hit
ACTTAAAAACAAGGCAGCTCTGAAACGGTATTTTTCGTGAGACCAAGTCT70.2057008521892448No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTCGTGAGACCAAGTCTCT60.17631501616220982No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC50.14692918013517484No Hit
ACTTAAAAACAAGGCAGTGTTAATGCCTAATGGTGCTACAGTTTCTGCCT50.14692918013517484No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT50.14692918013517484No Hit
TGTGTGTGTGTTTCTGTGGGCTTCTTTAAGGTTTGGACAGAAGGGTAAAG50.14692918013517484No Hit
TGTGTGTGTGTTTCTGTGGATTTCTTTAAGGTTTGGACAGAAGGGTAAAG50.14692918013517484No Hit
ACTTAAAAACAAGGCAGTTCTGACGGTATTTTTCGTGAGACCAAGTCTCT50.14692918013517484No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG40.11754334410813988No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTCGTGAGACCAAGTCTCT40.11754334410813988No Hit
CTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC40.11754334410813988No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCCACAGTTTCTGCCT40.11754334410813988No Hit
ACTTAAAAACAAGGCAGTTCTAATGCCTAATGGTGCTACAGTTTCTGCCT40.11754334410813988No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCCGCCT40.11754334410813988No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACGGAAGGGTAAAG40.11754334410813988No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTCAAGGTTTGGACAGAAGGGTAAAG40.11754334410813988No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)