FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3403
Total Bases493.2 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC77322.71525124889803No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC74021.745518660005878No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC66619.57096679400529No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG37511.019688510138113No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG1965.759623861298854No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG190.5583308845136644No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT150.44078754040552454No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.44078754040552454No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA140.4114017043784896No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.35263003232441964No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.32324419629738466No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT100.29385836027034967No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.23508668821627976No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.23508668821627976No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.23508668821627976No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.23508668821627976No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.23508668821627976No Hit
AAATACCGTTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAAC80.23508668821627976No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT80.23508668821627976No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.2057008521892448No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.2057008521892448No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT70.2057008521892448No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.2057008521892448No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT70.2057008521892448No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT70.2057008521892448No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.17631501616220982No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.17631501616220982No Hit
GGCACTAGAATGGACCTCTTCTCATCACTACATTATTTCAATTAAATACA60.17631501616220982No Hit
AAATACCGTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT50.14692918013517484No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT50.14692918013517484No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT50.14692918013517484No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT50.14692918013517484No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA50.14692918013517484No Hit
AAATACCGTTCAGAGCGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT50.14692918013517484No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.11754334410813988No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.11754334410813988No Hit
GGCACTAGAATGGACCTCTTCCTCATAACTACATTATTTCAATTAAATAC40.11754334410813988No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.11754334410813988No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.11754334410813988No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTAAATTAAATAC40.11754334410813988No Hit
AAATACCGTTCAGGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.11754334410813988No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGTTTGCAAATTGCTGCTGGAACC40.11754334410813988No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC40.11754334410813988No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)