FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5670
Total Bases822 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG203635.908289241622576No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT95916.913580246913583No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC92916.384479717813054No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC5339.400352733686066No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC3446.067019400352733No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG621.09347442680776No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC490.8641975308641975No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT240.4232804232804233No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC220.3880070546737213No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTCGTGAGACCAAGTCTCT200.3527336860670194No Hit
ACTTAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT150.26455026455026454No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC150.26455026455026454No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTCGTGAGACCAAGTCTCT100.1763668430335097No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT90.15873015873015872No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTCGTGAGACCAAGTCTCT90.15873015873015872No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC90.15873015873015872No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC80.14109347442680778No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG80.14109347442680778No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCTT80.14109347442680778No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT80.14109347442680778No Hit
ACTTAAAAACAAGGCAGCTCGAACGGTATTTTTCGTGAGACCAAGTCTCT70.12345679012345678No Hit
TGTGTGTGTGTTTCTGTGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC70.12345679012345678No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTCGTGAGACCAAGTCTCT70.12345679012345678No Hit
ACTTAAAAACAAGGCAGCTCTGAAACGGTATTTTTCGTGAGACCAAGTCT70.12345679012345678No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAGGGTTTGGACAGAAGGGTAAAG70.12345679012345678No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTGAAG60.10582010582010583No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAA60.10582010582010583No Hit
TGTGTGTGTGTTTTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC60.10582010582010583No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT60.10582010582010583No Hit
ACTTAAAAACAAGGCAGCTCTGAAGGTATTTTTCGTGAGACCAAGTCTCT60.10582010582010583No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACGGAAGGGTAAAG60.10582010582010583No Hit
TGTGTGTGTGTTTCTGTGGCAGCTCTGAACGGTATTTTTCGTGAGACCAA60.10582010582010583No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)