FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5670
Total Bases821.9 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC158027.86596119929453No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG92616.331569664902997No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC88015.520282186948853No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC5028.853615520282187No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG3436.049382716049383No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC2354.144620811287478No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT611.0758377425044092No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG440.7760141093474426No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.4409171075837742No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT240.4232804232804233No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.3703703703703704No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT200.3527336860670194No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA190.3350970017636684No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT180.31746031746031744No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA170.2998236331569665No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.28218694885361556No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA150.26455026455026454No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.22927689594356263No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA130.22927689594356263No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.19400352733686066No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT110.19400352733686066No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT100.1763668430335097No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.1763668430335097No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT90.15873015873015872No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT90.15873015873015872No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA90.15873015873015872No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT80.14109347442680778No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA80.14109347442680778No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.14109347442680778No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.14109347442680778No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT80.14109347442680778No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT70.12345679012345678No Hit
AAATACCGTTCGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT70.12345679012345678No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT70.12345679012345678No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTAAATTAAATAC70.12345679012345678No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC60.10582010582010583No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC60.10582010582010583No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC60.10582010582010583No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAAAGACGTGTAGAA60.10582010582010583No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.10582010582010583No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTAGCAAATTGCTGCTGGAACC60.10582010582010583No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC60.10582010582010583No Hit
AAATACCTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT60.10582010582010583No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)