FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5659
Total Bases820.4 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG205736.34917830005301No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC161328.503269128821344No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC84814.984979678388408No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC2915.142251281145079No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG801.4136773281498498No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC460.8128644636861637No Hit
ACTTAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT380.6714967308711787No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTCGTGAGACCAAGTCTCT250.4417741650468281No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT200.35341933203746245No Hit
ACTTAAAAACAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT200.35341933203746245No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC140.24739353242622372No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG130.2297225658243506No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG100.17670966601873123No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT90.1590386994168581No Hit
ACTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT90.1590386994168581No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCCGCCTC90.1590386994168581No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC90.1590386994168581No Hit
TGTTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC80.141367732814985No Hit
ACTTAAAAACAAGGCAGTGCTGATGCTAATGGTGCTACAGTTTCTGCCTC80.141367732814985No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT80.141367732814985No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGGAGGGTAAAG70.12369676621311186No Hit
ACTTAAAAACAAGGCAGTTCTAATGCTAATGGTGCTACAGTTTCTGCCTC70.12369676621311186No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGTTACAGTTTCTGCCTC70.12369676621311186No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT70.12369676621311186No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTCGTGAGACCAAGTCTCT60.10602579961123874No Hit
ACTTAAAAACAAGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT60.10602579961123874No Hit
TGTGTGGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC60.10602579961123874No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG60.10602579961123874No Hit
ATTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT60.10602579961123874No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACGGTTTCTGCCTC60.10602579961123874No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACAAAGTCTC60.10602579961123874No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG60.10602579961123874No Hit
ACTTAAAAACAAGGCAGCTCTGAAACGGTATTTTTCGTGAGACCAAGTCT60.10602579961123874No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)