FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630
Total Bases91.3 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC13120.793650793650794No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC12019.047619047619047No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC10917.3015873015873No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG7311.587301587301587No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG416.507936507936509No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT193.015873015873016No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC132.0634920634920633No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG50.7936507936507936No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.4761904761904762No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT30.4761904761904762No Hit
AAATACCGTTCAGAGCTGCCACAAGGAGCTTGCAAATTGCTGCTGGAACC30.4761904761904762No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.4761904761904762No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.31746031746031744No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCAGGAACC20.31746031746031744No Hit
AAATACCGTTCAGAGTTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC20.31746031746031744No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.31746031746031744No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.31746031746031744No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA20.31746031746031744No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT20.31746031746031744No Hit
AAATACCGTTCAGAGCTGCCACCAAGCGCTTGCAAATTGCTGCTGGAACC20.31746031746031744No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.31746031746031744No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC20.31746031746031744No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT20.31746031746031744No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT20.31746031746031744No Hit
AAATACCGTTCAGAACTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC20.31746031746031744No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.31746031746031744No Hit
AAATACAGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC10.15873015873015872No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.15873015873015872No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATCGCTGCTGGAACC10.15873015873015872No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAACTGCTGCTGGAACC10.15873015873015872No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA10.15873015873015872No Hit
AAATACCGTCCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.15873015873015872No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTACATTATTTCAATTAAATAC10.15873015873015872No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTTCTGGAACC10.15873015873015872No Hit
AAATACCGTTCGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.15873015873015872No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAAATAAATAC10.15873015873015872No Hit
AAATACCGTTCAGAGCAGCCACCAGCAGCTTTCAAATTGATGAAGGAACC10.15873015873015872No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCCACAGAAACAC10.15873015873015872Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GGCACTAGATTGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC10.15873015873015872No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACA10.15873015873015872No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAATAAGACGTGTAGAACCATG10.15873015873015872No Hit
AAATACCGTTCAGAGCTCCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.15873015873015872No Hit
GGCACTAGAATGGACATCTTCCTCATCACTACATTATTTCAATTAAATAC10.15873015873015872No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.15873015873015872No Hit
AAAAACCGTTCAGAGCTCCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.15873015873015872No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCCTGCAAATTGCTGCTGGAACC10.15873015873015872No Hit
AAATACCGTTAAGAGCTGCCACCAGGAGCTTGCAAACTGCTGCTGGAACC10.15873015873015872No Hit
GCACTAGATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAAGA10.15873015873015872No Hit
AAATACCGTTCAGAGCTCCCAGCAGGAGCTAGCAAATAGCAGCTGGAACC10.15873015873015872No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAATAAGACGTGTAGAACCATGT10.15873015873015872No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGAAACC10.15873015873015872No Hit
AAATTCCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC10.15873015873015872No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.15873015873015872No Hit
AAATACCGTTCAGCACTGCCTGTTTTTAAGTAAGACGTGTAGAACCATGT10.15873015873015872No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.15873015873015872No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTTGAACCT10.15873015873015872No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGTACAGCACT10.15873015873015872No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC10.15873015873015872No Hit
AAATACCGTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.15873015873015872No Hit
AAACGTTCAGAGCTGCCACCAAGAGCTAGCAAATTGCTGCTGCAACCTGA10.15873015873015872No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.15873015873015872No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA10.15873015873015872No Hit
AAAACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCTG10.15873015873015872No Hit
AAATACCGTTCAGAGCTGCCTGGAGGCCGTGGCCACATGACCCTGGGCCG10.15873015873015872No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCGCAGCACT10.15873015873015872No Hit
AAATACCGTTCAGAGCTCCCACCAGGAGCTTCCAAATTGATGATGGAACA10.15873015873015872No Hit
AAATACCGTTCAGCACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT10.15873015873015872No Hit
AATACCGTTCAGAGCTGACACCAGGAGCTTGCAAATTGATGCTGGAACCT10.15873015873015872No Hit
GGCATAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACAA10.15873015873015872No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.15873015873015872No Hit
AAATACCGTTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAAC10.15873015873015872No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCGATTAAATAC10.15873015873015872No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACAGTATTTCAATTAAATAC10.15873015873015872No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGTTTGCAAATTGCTGCTGGAACC10.15873015873015872No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.15873015873015872No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACAATATTTCAATTAAATAC10.15873015873015872No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.15873015873015872No Hit
GGTACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAACTAAATAC10.15873015873015872No Hit
GGCACTGGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC10.15873015873015872No Hit
AAATACCGTTCATAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.15873015873015872No Hit
AAATACCTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.15873015873015872No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGAAAATTGCTGCTGGAACC10.15873015873015872No Hit
AATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGTC10.15873015873015872No Hit
GGCACTAGAATGGACCTCTTCATCATCACTACATTATTTCAATTAAATAC10.15873015873015872No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTACCCACAGAAACA10.15873015873015872Clontech Universal Primer Mix Long (95% over 23bp)
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA10.15873015873015872No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAACTGCTGCTGGAACC10.15873015873015872No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.15873015873015872No Hit
AAATACCGTTCATAGCTGCCACCAAGAGCTTTCAAATTGCTGCTGGAAAC10.15873015873015872No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGAAAATTGCTGCAGGAACA10.15873015873015872No Hit
GGCGCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC10.15873015873015872No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.15873015873015872No Hit
GGCACTAGATAGACCTCTTCCTCATCACTACGTTATTTCAATTAAATACA10.15873015873015872No Hit
AAATCCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.15873015873015872No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGATCC10.15873015873015872No Hit
AAATACCGTTCAGAGCTGCCACCAGGGGCTTGCAAATTGCTGCTGGAACC10.15873015873015872No Hit
AGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC10.15873015873015872No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAGTAC10.15873015873015872No Hit
AAATACCGTTCAGGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.15873015873015872No Hit
GACACTAGAATGGACCTCTTCGTTGATACCACTGCTTACCCACAGAAACA10.15873015873015872Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.15873015873015872No Hit
AAATACCGTTCAGAGCTGCCACCGGGAGCTTGCAAATTGCTGCTGGAACC10.15873015873015872No Hit
TGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC10.15873015873015872No Hit
AAATACCGTTCAGAGCTTCCACCAGGAGCTTGCAAAATCCTGCTGGAACC10.15873015873015872No Hit
AAATACGTTCAGAGCTGCCACCAGGAGTTTGCAAATTGCTGCTGGAACCT10.15873015873015872No Hit

[FAIL]Adapter Content

Adapter graph