FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4355
Total Bases631.4 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT138531.802525832376578No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG127729.322617680826635No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC79018.14006888633754No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC2766.337543053960965No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC481.1021814006888633No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC400.9184845005740528No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTCGTGAGACCAAGTCTCT170.39035591274397247No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT170.39035591274397247No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC120.2755453501722158No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT110.25258323765786456No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT100.2296211251435132No Hit
ACTTAAAAACAAGGCAGCTCTGAAACGGTATTTTTCGTGAGACCAAGTCT100.2296211251435132No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC90.20665901262916186No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG80.18369690011481057No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTCGTGAGACCAAGTCTCT80.18369690011481057No Hit
TGTGTGTGTGTTTCTTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC80.18369690011481057No Hit
TGTGTGGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC80.18369690011481057No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC80.18369690011481057No Hit
ACTTAAAAACAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA60.1377726750861079Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGTTCTGAAACGGTATTTTTCGTGAGACCAAGTCT60.1377726750861079No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC60.1377726750861079No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC60.1377726750861079No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTCGTGAGACCAAGTCTCT50.1148105625717566No Hit
ACTTAAAAACAAGGCAGTGCTAATGACTAATGGTGCTACAGTTTCTGCCT50.1148105625717566No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGTCT50.1148105625717566No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTGCCTT50.1148105625717566No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTCGTGAGACCAAGTCTCT50.1148105625717566No Hit
ACTTAAAAACAAGGCAGTGCTAATGTCTAATGGTGCTACAGTTTCTGCCT50.1148105625717566No Hit
ACTTAAAAACAAGGAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC50.1148105625717566No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)