FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605
Total Bases87.7 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT25441.98347107438016No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC12420.49586776859504No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG9615.867768595041323No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC477.768595041322314No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC91.487603305785124No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG81.322314049586777No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC71.1570247933884297No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTCGTGAGACCAAGTCTCT30.49586776859504134No Hit
ACTTAAAAACAAGGCGGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT30.49586776859504134No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC20.3305785123966942No Hit
TGTGTGTGTGTTTCTGTGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC20.3305785123966942No Hit
ATTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT20.3305785123966942No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC20.3305785123966942No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTGTCTGCCT10.1652892561983471No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTCGTGAGACCAAGTCTCT10.1652892561983471No Hit
ACTTAAAAACAAGGCAGCTCGAACGGTATTTTTCGTGAGACCAAGTCTCT10.1652892561983471No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC10.1652892561983471No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.1652892561983471No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.1652892561983471No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGATACAGTTTCTGCCT10.1652892561983471No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT10.1652892561983471No Hit
ACTTAAAAACAAGGCAGTGCTAACGGTATTTTTCGTGAGACCAAGTCTCT10.1652892561983471No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGCAAAG10.1652892561983471No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGATGCTACAGTTTCTGCCT10.1652892561983471No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGTTACAGTTTCTGCCT10.1652892561983471No Hit
ACATAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT10.1652892561983471No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCAT10.1652892561983471No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACGGTTTCTGCCT10.1652892561983471No Hit
ACTTAAAAACAAGGCAGCTCTGACGTATTTTTCGTGAGACCAAGTCTCTG10.1652892561983471No Hit
ACTAAAAACAGGCAGTGCTGATGCCTAATGGTGCTACAGTTTCTGCCTCT10.1652892561983471No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC10.1652892561983471No Hit
TGTGGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.1652892561983471No Hit
ACTTAAAAACAAGGCAGTTCTGAAACGGTATTTTTCGTGAGACCAAGTCT10.1652892561983471No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAGAG10.1652892561983471No Hit
ACTTAAAAACAAGGCAGTGCTAATACCTAATGGCGCTACAGTTTCTGCCT10.1652892561983471No Hit
TGTGTGTGTGTTTCTGTGGTTTTCTTTAAGGTTTGGACAGAAGGGTAAAG10.1652892561983471No Hit
ACTTAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTCT10.1652892561983471No Hit
ACTTAAAAACAAGGCAGTGCTAGTGCCTAATGGTGCTACAGTTTCTGCCT10.1652892561983471No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGGGCTACAGTTTCTGCCTC10.1652892561983471No Hit
ACTTAAAAACAAGGCAGTTCTAATGCCTAATGGTGCTACAGTTTCTGCCT10.1652892561983471No Hit
ACTTAAAAACAGGCAGTGCTAATACCTAATGGTGCTACAGTTTCTGCCTC10.1652892561983471No Hit
TGTGTGTGGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.1652892561983471No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.1652892561983471No Hit
TGGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.1652892561983471No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACACAAGGGTAAAG10.1652892561983471No Hit
ACTTAAAAACAAGGCAGCTCTGAAACGGTATTTTTCGTGAGACCAAGTCT10.1652892561983471No Hit
TGAGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG10.1652892561983471No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGTGCTACAGTTTCTGCCTC10.1652892561983471No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGATAGAAGGGTAAAG10.1652892561983471No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTCGTGAGACCAAGTCTCTG10.1652892561983471No Hit
ACTTAAAAACAAGGCATGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC10.1652892561983471No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTAGTGAGACCAAGTCTC10.1652892561983471No Hit
ACTTAAAAACAAGGCGGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.1652892561983471No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTTGACAGAAGGGTAAAG10.1652892561983471No Hit
ACTTAAAAACAAGGCAGTGCTGAACGTATTTTTCGTGAGACCAAGTCTCT10.1652892561983471No Hit
ACTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.1652892561983471No Hit
ACTTAAAAACAAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT10.1652892561983471Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTTTGCCT10.1652892561983471No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAACACTC10.1652892561983471No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)