FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476
Total Bases213.9 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC32321.883468834688347No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC25817.479674796747968No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC21714.70189701897019No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG18212.330623306233063No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC1147.723577235772358No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG714.8102981029810294No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT191.2872628726287263No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG181.2195121951219512No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT100.6775067750677507No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.47425474254742545No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.47425474254742545No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.40650406504065045No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.40650406504065045No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT60.40650406504065045No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.27100271002710025No Hit
AAATCCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT40.27100271002710025No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.27100271002710025No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.27100271002710025No Hit
AAATACCGTTCAGAGTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.20325203252032523No Hit
AAATACCTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.20325203252032523No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.20325203252032523No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.20325203252032523No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.20325203252032523No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.20325203252032523No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT30.20325203252032523No Hit
GGCACTAGAATGGACCTCTTCTCATCACTAAATTATTTCAATTAAATACA30.20325203252032523No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTAAGACGTGTAGAACCATGT20.13550135501355012No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.13550135501355012No Hit
GGCACTAGAATGGACCTCTTCCTCATCACCACATTATTTCAATTAAATAC20.13550135501355012No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT20.13550135501355012No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC20.13550135501355012No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.13550135501355012No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAGCC20.13550135501355012No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.13550135501355012No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTAAATTATTTCAATTAAATAC20.13550135501355012No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC20.13550135501355012No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.13550135501355012No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATCAAATAC20.13550135501355012No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.13550135501355012No Hit
GGCACTAGAATGGACCTCTTCCTCATCGCTACATTATTTCAATTAAATAC20.13550135501355012No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.13550135501355012No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT20.13550135501355012No Hit
AAATACCGTTCAGGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT20.13550135501355012No Hit
AAATACCGTTCAGACTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT20.13550135501355012No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT20.13550135501355012No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA20.13550135501355012No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.13550135501355012No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT20.13550135501355012No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)