FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2835
Total Bases411 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG103836.613756613756614No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT86330.440917107583775No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC32311.393298059964726No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC1705.996472663139329No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG280.9876543209876543No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC270.9523809523809524No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC270.9523809523809524No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC110.3880070546737213No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTCGTGAGACCAAGTCTCT100.3527336860670194No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC80.28218694885361556No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTCGTGAGACCAAGTCTCT70.24691358024691357No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT60.21164021164021166No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG50.1763668430335097No Hit
TGTGGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC50.1763668430335097No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC50.1763668430335097No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG50.1763668430335097No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC40.14109347442680778No Hit
ACTTAAAAACAAGGCAGTGTTAATGCCTAATGGTGCTACAGTTTCTGCCT40.14109347442680778No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCCACAGTTTCTGCCT40.14109347442680778No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTCGTGAGACCAAGTCTCT40.14109347442680778No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGGTCCATTCTAGTGCCTT40.14109347442680778Clontech Universal Primer Mix Long (95% over 22bp)
ACTTAAAAACAAGGCAGTTCTAATGCCTAATGGTGCTACAGTTTCTGCCT40.14109347442680778No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT40.14109347442680778No Hit
ACTTAAAAACAAGGCAGTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT40.14109347442680778No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC40.14109347442680778No Hit
ACTTAAAAACAAGGCAGCTCTGAAACGGTATTTTTCGTGAGACCAAGTCT40.14109347442680778No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGTTACAGTTTCTGCCT30.10582010582010583No Hit
TTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC30.10582010582010583No Hit
TGTGTGTTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC30.10582010582010583No Hit
ACTTAAAAACAAGGCAGTTCTAACGGTATTTTTCGTGAGACCAAGTCTCT30.10582010582010583No Hit
ACTTAAAAACAAGGCAGTGCTGATGCCTAATGGTGCTACAGTTTCTGCCT30.10582010582010583No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGCAAAG30.10582010582010583No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT30.10582010582010583No Hit
TGTGTGTGTGTTTCTGTGGGCTTCTTTAAGGTTTGGACAGAAGGGTAAAG30.10582010582010583No Hit
TGTGTGTGTGTTTCTGTGGATTTCTTTAAGGTTTGGACAGAAGGGTAAAG30.10582010582010583No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAGTGGTGCTACAGTTTCTGCCT30.10582010582010583No Hit
ACTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT30.10582010582010583No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG30.10582010582010583No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC30.10582010582010583No Hit
ACTTAAAAACAAGGCAGTTCTGACGGTATTTTTCGTGAGACCAAGTCTCT30.10582010582010583No Hit
ATTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT30.10582010582010583No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTCGTGAGACCAAGTCTCT30.10582010582010583No Hit
ATTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC30.10582010582010583No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAGGGTTTGGACAGAAGGGTAAAG30.10582010582010583No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTGCAGTTTCTGCCT30.10582010582010583No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)