FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022
Total Bases148.1 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT47946.86888454011742No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG17116.731898238747554No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC13713.405088062622308No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC494.794520547945205No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG333.2289628180039136No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC191.8590998043052838No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC90.8806262230919765No Hit
ACTTAAAAACAAGGCAGTGTTAATGCCTAATGGTGCTACAGTTTCTGCCT40.3913894324853229No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTCGTGAGACCAAGTCTCT30.29354207436399216No Hit
ACTTAAAAACAAGGCAGCTCTGAACGTATTTTTCGTGAGACCAAGTCTCT30.29354207436399216No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC30.29354207436399216No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT30.29354207436399216No Hit
ACTTAAAAACAAGGCCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCC20.19569471624266144No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGTTACAGTTTCTGCCT20.19569471624266144No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGGTCCATTCTAGTGCCTT20.19569471624266144Clontech Universal Primer Mix Long (95% over 22bp)
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGTCT20.19569471624266144No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTACCT20.19569471624266144No Hit
ACTTAAAAACAAGGCATGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC20.19569471624266144No Hit
ACTTAAAAACAAGGCAGTGCTAAAGCCTAATGGTGCTACAGTTTCTGCCT20.19569471624266144No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC20.19569471624266144No Hit
ACTTAAAAACAAGGTAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT20.19569471624266144No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT20.19569471624266144No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC20.19569471624266144No Hit
ACTTAAAAACAAGGCAGTGCTAGTGCCTAATGGTGCTACAGTTTCTGCCT20.19569471624266144No Hit
ACTTAAAAACAAGGAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC20.19569471624266144No Hit
ACTTAAAAACAAGGCAGCTCTGAAACGGTATTTTTCGTGAGACCAAGTCT20.19569471624266144No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTCGTGAGACCAAGTCTCT20.19569471624266144No Hit
ACTTAAAAACAAGGCAGCCTGAACGGTATTTTTCGTGAGACCAAGTCTCT20.19569471624266144No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)