FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391
Total Bases56.6 kbp
Sequences flagged as poor quality0
Sequence length145
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG18346.80306905370844No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC7519.18158567774936No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC4310.997442455242968No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT379.462915601023019No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC51.278772378516624No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT41.0230179028132993No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG30.7672634271099744No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTCGTGAGACCAAGTCTCT20.5115089514066496No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC20.5115089514066496No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG20.5115089514066496No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT20.5115089514066496No Hit
TGTGGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC20.5115089514066496No Hit
ACTTAAAAACAAGGCAGTTCTAACGGTATTTTTCGTGAGACCAAGTCTCT10.2557544757033248No Hit
ACTTAAAAAAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.2557544757033248No Hit
CTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC10.2557544757033248No Hit
TGTGTTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.2557544757033248No Hit
TGTGTGTGTGTTTTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.2557544757033248No Hit
TGTGTGTGTGTTTCTTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.2557544757033248No Hit
ACTTAAAAACAAGGCAGCTCGAACGGTATTTTTCGTGAGACCAAGTCTCT10.2557544757033248No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACAAAGTCTC10.2557544757033248No Hit
TTTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT10.2557544757033248No Hit
ACTTAAAAACAAGGCAGTGCTGATGCCTAATGGTGCTACAGTTTCTGCCT10.2557544757033248No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG10.2557544757033248No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTATGGACAGAAGGGTAAAG10.2557544757033248No Hit
ACTTAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.2557544757033248No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGAAGAGGTCCATTCTAGTACCTT10.2557544757033248No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.2557544757033248No Hit
ACTTAAAAACAAGGCAGCTCTGAAACGGTATTTTTCGTGAGACCAAGTCT10.2557544757033248No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGCCCAAGTCTC10.2557544757033248No Hit
ACTTAAAAACAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.2557544757033248No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGATACAGTTTCTGCCT10.2557544757033248No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC10.2557544757033248No Hit
TGTGTGTGTTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.2557544757033248No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTGCAGTTTCTGCCT10.2557544757033248No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTGAGGTTTGGACAGAAGGGTAAAG10.2557544757033248No Hit
TGTGTGTGTGTTTCTGTGGGTAAGCAGTGGTATCAACGCAGAGTACATGG10.2557544757033248Clontech Universal Primer Mix Long (96% over 27bp)
ACTTAAAAACAAGGCAGTCCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.2557544757033248No Hit
TGTGTGCGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG10.2557544757033248No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTCGTGAGACCAAGTCTCT10.2557544757033248No Hit
TTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.2557544757033248No Hit
TGTGTGGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.2557544757033248No Hit
ACTTAAAAACACGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT10.2557544757033248No Hit
TGTGTGTTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.2557544757033248No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)