FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3151
Total Bases456.7 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC91629.07013646461441No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC54717.359568390986986No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG32510.314185972707076No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC2899.171691526499524No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC2558.092668993970168No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG1023.2370675975880667No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT872.7610282450015866No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA230.7299270072992701No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT190.6029831799428753No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG150.47603935258648045No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT140.4443033957473818No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.3808314820691844No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.31735956839098695No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT90.2856236115518883No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.25388765471278957No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT80.25388765471278957No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.25388765471278957No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT80.25388765471278957No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA80.25388765471278957No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.2221516978736909No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.2221516978736909No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.2221516978736909No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA60.1904157410345922No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.1904157410345922No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.1904157410345922No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.1904157410345922No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.1904157410345922No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.15867978419549347No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT50.15867978419549347No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.15867978419549347No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.12694382735639478No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.12694382735639478No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT40.12694382735639478No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.12694382735639478No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT40.12694382735639478No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.12694382735639478No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.12694382735639478No Hit
GGCACTAGAATGGACCTCGTCCTCATCACTACATTATTTCAATTAAATAC40.12694382735639478No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT40.12694382735639478No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT40.12694382735639478No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC40.12694382735639478No Hit
AATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACTG40.12694382735639478No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.12694382735639478No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)