FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504
Total Bases73 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC16632.93650793650794No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG15530.753968253968257No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG7314.484126984126986No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT61.1904761904761905No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG61.1904761904761905No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.992063492063492No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT40.7936507936507936No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.7936507936507936No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.7936507936507936No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.5952380952380952No Hit
GGCACTAGAATGGACCTCTTCAGAGCAACTCTGCGTTGATACCACTGCTT30.5952380952380952Clontech SMART CDS Primer II A (95% over 24bp)
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.5952380952380952No Hit
AAATACCGTTAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.3968253968253968No Hit
GGCACTAGAATGGATCTCTTCCTCATCACTACATTATTTCAATTAAATAC20.3968253968253968No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.3968253968253968No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.3968253968253968No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT20.3968253968253968No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTCCCATGTACTCTGC20.3968253968253968Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GTCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC10.1984126984126984No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTTAAAAC10.1984126984126984No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTCCCATGTACTCTGC10.1984126984126984Clontech Universal Primer Mix Long (95% over 22bp)
AAATACCGTTCAGCGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG10.1984126984126984No Hit
GACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACAA10.1984126984126984No Hit
AATACCGTTGAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.1984126984126984No Hit
GGCACAGAATGGACCTCTTCCTCAACAATACATTATTTCAATTAAATACA10.1984126984126984No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTGCATGTACTCTGCG10.1984126984126984Clontech SMARTer II A Oligonucleotide (95% over 21bp)
AAATACCGTTCAGAGCTGCCTTGTTTTTAAAAGACGTGTAGAACCATGTC10.1984126984126984No Hit
GGCACTAGAATGGACCTCTTCAGAGCAACTCTGCGTTGATACCACTGCTC10.1984126984126984Clontech SMART CDS Primer II A (95% over 23bp)
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.1984126984126984No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.1984126984126984No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.1984126984126984No Hit
GGCACTAGAATGGACCTCGTCCTCATCACTACATTATTTCAATTAAATAC10.1984126984126984No Hit
AAATACCGTTCAGAGCTGCCTCGTTTTTAAGTAAGACGTGTAGAACCATG10.1984126984126984No Hit
GGCACTAGATGGACCTCTTCCTCTTCACTACATTATTTCAATTAAATACA10.1984126984126984No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTTGTTTTTAAGTAA10.1984126984126984Clontech Universal Primer Mix Long (95% over 23bp)
GGCATAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACAA10.1984126984126984No Hit
GGCACAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACAA10.1984126984126984No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAAAGACGTGTAGAA10.1984126984126984No Hit
GGCACTAGAATGGACCTCGTCCTCATCACTACATTATTTAAATTAAATAT10.1984126984126984No Hit
AAATAGTTCAGAGCTGCCACAGAAACACACACACAAAGACGTGTAGAACC10.1984126984126984No Hit
AAATACCGTTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT10.1984126984126984No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC10.1984126984126984No Hit
GGCACTAGAATGGACCTCTTCAGAGCAACTCTGCGTTGATAGCACTGCCT10.1984126984126984Clontech Universal Primer Mix Long (95% over 21bp)
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTCCATGTACTCTGC10.1984126984126984Clontech Universal Primer Mix Long (95% over 23bp)
GGCACTAGAATGGACCTCTTCCTCATCACAACATTATTTCAAATAAATAC10.1984126984126984No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCTG10.1984126984126984No Hit
GGCACTAGAATGGACCCCACAGAAACACACACACAAAGACGTGTAGAACC10.1984126984126984No Hit
GGCAGTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC10.1984126984126984No Hit
AAATACCGTTCCGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG10.1984126984126984No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.1984126984126984No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.1984126984126984No Hit
AAATACCGTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.1984126984126984No Hit
GGCACTAGAATGGACCTCTTCCTCATCAATACATTATTTCAATTAAATAC10.1984126984126984No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATTC10.1984126984126984No Hit
GGCACTAGAATGGACCTCATCCTCATAACTACATTATTTCAATTAAATAC10.1984126984126984No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC10.1984126984126984No Hit
AAATACCGTTCAGAGTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.1984126984126984No Hit
GGCACTAAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACAA10.1984126984126984No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.1984126984126984No Hit
GGCACTAGAATGGACCTCTTCTGCGTTGATACCACTGTCCCATGTACTCT10.1984126984126984No Hit
AAATACCGTTCAGAGCTGCCTTGTGTTTAAGTAAGACGTGTAGAACCATG10.1984126984126984No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAGTAAGACGTGTAGAACCATGTC10.1984126984126984No Hit
GGCACTAGAATGGACCTCTTTCTCATCAATACATTATTTCAATTAAATAC10.1984126984126984No Hit
AAATACCGTTCAGAGCTGCCATGTTTTTAAGTAAGACGTGTAGAACCATG10.1984126984126984No Hit
GGACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACAA10.1984126984126984No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTACTTCAATTAAATAC10.1984126984126984No Hit
AAATACCGTTCAGAACTGCCTGTTTTTAAGTAAGACGTGTAGAACCATGT10.1984126984126984No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCTGT10.1984126984126984No Hit
GGCACTAGAAAGGACCTCTTCCTCATCACTAAATTATATCAATAAAATAC10.1984126984126984No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAAGT10.1984126984126984No Hit
AAATACCGTACAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG10.1984126984126984No Hit
AAATACCGTTCGGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG10.1984126984126984No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT10.1984126984126984No Hit
AAATACCGTTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCA10.1984126984126984No Hit
GGCACTAAATGGACCTCTTCGTTGATACCACTGCTGCACTGCCTTGTTTT10.1984126984126984Clontech SMARTer II A Oligonucleotide (95% over 21bp)
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.1984126984126984No Hit
AAATACCGTTCAGAACTGCCTTTTTTTAAGTAAGACGTGTAGAACCATGT10.1984126984126984No Hit
AATACCTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGTC10.1984126984126984No Hit

[FAIL]Adapter Content

Adapter graph