FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4694
Total Bases680.4 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC116924.90413293566255No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC70615.040477204942478No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG61613.123135918193437No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC54411.589262888794206No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC3918.329782701320836No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG2304.89987217724755No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT1022.1729867916489134No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA250.5325948018747336No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG210.44737963357477634No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT200.42607584149978694No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT190.4047720494247976No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA160.34086067319982954No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT160.34086067319982954No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.27694929697486154No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT130.27694929697486154No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.2556455048998722No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.2343417128248828No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.2343417128248828No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.21303792074989347No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.21303792074989347No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT90.19173412867490414No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.19173412867490414No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.19173412867490414No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTAAATTATTTCAATTAAATAC80.17043033659991477No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT80.17043033659991477No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA70.14912654452492544No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.14912654452492544No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.14912654452492544No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT70.14912654452492544No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.14912654452492544No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.14912654452492544No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.1278227524499361No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGAAAGCCAATGGGAAA60.1278227524499361No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.1278227524499361No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT60.1278227524499361No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA60.1278227524499361No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.10651896037494674No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.10651896037494674No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.10651896037494674No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAAAGACGTGTAGAA50.10651896037494674No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)