FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083
Total Bases157 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC44140.7202216066482No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG25423.4533702677747No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG756.9252077562326875No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC433.970452446906741No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT171.569713758079409No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG161.477377654662973No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT151.3850415512465373No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.6463527239150507No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC70.6463527239150507No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA70.6463527239150507No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.554016620498615No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT60.554016620498615No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT50.46168051708217916No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT50.46168051708217916No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTGC50.46168051708217916Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.3693444136657433No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCGAATTGCTGCTGGAACC40.3693444136657433No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC30.2770083102493075No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAGTAAGACGTGTAGAACCATGT30.2770083102493075No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.2770083102493075No Hit
AAATACCGTTCGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.18467220683287164No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAAAGACGTGTAGAA20.18467220683287164No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.18467220683287164No Hit
AAATACCGTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.18467220683287164No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCCGCTGGAACC20.18467220683287164No Hit
AAATACCGTTCAGGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.18467220683287164No Hit
AAATACCGTTCAGAGTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.18467220683287164No Hit
AAATACTGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC20.18467220683287164No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGTTGCTGGAACC20.18467220683287164No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTCGCAAATTGCTGCTGGAACC20.18467220683287164No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGTTTGCAAATTGCTGCTGGAACC20.18467220683287164No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTACTGCTGGAACC20.18467220683287164No Hit
AAATCCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.18467220683287164No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.18467220683287164No Hit
AAATACCTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.18467220683287164No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACATGT20.18467220683287164No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.18467220683287164No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT20.18467220683287164No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.18467220683287164No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGGACC20.18467220683287164No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA20.18467220683287164No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTACTGGAACC20.18467220683287164No Hit
AAATACCGTTCAGAGCTGCCACCAGTAGCTTGCAAATTGCTGCTGGAACC20.18467220683287164No Hit

[FAIL]Adapter Content

Adapter graph