FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5706
Total Bases826.9 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC142324.938661058534876No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC138724.307746232036454No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG57810.12968804766912No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC5058.850332982825096No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC4477.833859095688749No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG1843.224675779880827No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG460.8061689449702067No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA250.43813529617946023No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA210.36803364879074657No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT210.36803364879074657No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT210.36803364879074657No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA200.3505082369435682No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA190.33298282509638977No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA180.31545741324921134No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.28040658955485454No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.28040658955485454No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT160.28040658955485454No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.24535576586049773No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.24535576586049773No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.2278303540133193No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT130.2278303540133193No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.2278303540133193No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.2103049421661409No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.1927795303189625No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.1927795303189625No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.1927795303189625No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.1752541184717841No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.1752541184717841No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA100.1752541184717841No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.15772870662460567No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.15772870662460567No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC90.15772870662460567No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.15772870662460567No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.14020329477742727No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT80.14020329477742727No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTAAATTATTTCAATTAAATAC70.12267788293024887No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT70.12267788293024887No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTACAATTAAATAC70.12267788293024887No Hit
AAATACCGTTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAAC70.12267788293024887No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.10515247108307045No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTAAATTATTTCAATTAAATAC60.10515247108307045No Hit
GGCACTAGAATGGACCTCTTCCTCACCACTACATTATTTCAATTAAATAC60.10515247108307045No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC60.10515247108307045No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.10515247108307045No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.10515247108307045No Hit
GGCACTAGAATGGACCTCTCCTCATCACTACATTATTTCAATTAAATACA60.10515247108307045No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACAATATTTCAATTAAATAC60.10515247108307045No Hit
GGCACTAGAATGGACCTCTTCTCATCACTAAATTATTTCAATTAAATACA60.10515247108307045No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)