FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510
Total Bases73.9 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC14227.84313725490196No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG8917.45098039215686No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC6713.137254901960786No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC499.607843137254903No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG448.627450980392156No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC122.3529411764705883No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT122.3529411764705883No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.5882352941176471No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.5882352941176471No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.5882352941176471No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.39215686274509803No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATAC20.39215686274509803No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.39215686274509803No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT20.39215686274509803No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.39215686274509803No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG20.39215686274509803No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC20.39215686274509803No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.39215686274509803No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT20.39215686274509803No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.39215686274509803No Hit
AAATACCGTTCAGAGTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.19607843137254902No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGAAGTGTAGAACCAAG10.19607843137254902No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.19607843137254902No Hit
AAAAACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT10.19607843137254902No Hit
GGACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACAA10.19607843137254902No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATCGCTGCTGGAACC10.19607843137254902No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGCACCATG10.19607843137254902No Hit
AAATACCGTTCAGAGCTCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.19607843137254902No Hit
AAATACCGTTCAGAGATGACTTGTTTTTAAGTAAGACGTGTATAACCATG10.19607843137254902No Hit
AAATACCGCTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC10.19607843137254902No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.19607843137254902No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGTTTGCAAATTGCTGCTGGAACC10.19607843137254902No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATAGCTGCTGGAACC10.19607843137254902No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATAATTTCAATTAAATAC10.19607843137254902No Hit
AAATCCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.19607843137254902No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC10.19607843137254902No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC10.19607843137254902No Hit
AACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCTGA10.19607843137254902No Hit
AAATCCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.19607843137254902No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT10.19607843137254902No Hit
AAATACCTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.19607843137254902No Hit
AAATACCGTTCAGAGCAGCCACCAAGAGCTTGAAAATTGCTGCTGGAACC10.19607843137254902No Hit
GGCACTAGAATGGACCTCTTCCTCAGCACTACAATATTTCAATTAAATAC10.19607843137254902No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.19607843137254902No Hit
AAATACAGTTCACAGCTGCCACCAAGAGCATGCAAATTGCTGCTGGAAAA10.19607843137254902No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCCGCTGGAACC10.19607843137254902No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.19607843137254902No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.19607843137254902No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA10.19607843137254902No Hit
GGCACTAGAATGGACCTCTTACTCATCACTACATTATTTCAATTAAATAC10.19607843137254902No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATCTCAATTAAATAC10.19607843137254902No Hit
AAATACCGTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.19607843137254902No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGATGGAACC10.19607843137254902No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA10.19607843137254902No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACGTTATTTCAATTAAATAC10.19607843137254902No Hit
GGCACTGATTGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.19607843137254902No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTCCCTGGGAGGGCCCAGCACT10.19607843137254902No Hit
ACATACCGTTCAGAGCTGCCCCCAGGAGCTTGCAAATTTCAGCTGGAACC10.19607843137254902No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTAAATTAAATAC10.19607843137254902No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCGCAGCACT10.19607843137254902No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.19607843137254902No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTATCTGGGAGGGCACAGCACT10.19607843137254902No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.19607843137254902No Hit
AAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.19607843137254902No Hit
AAATACCGTTCAGAGCTGCCACCAGGGGCTTGCAAATTGCTGCTGGAACC10.19607843137254902No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTTCTGCTGGAACC10.19607843137254902No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA10.19607843137254902No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGTTGGAACCT10.19607843137254902No Hit
GGACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACAA10.19607843137254902No Hit
AAATACCGTTCAGAGCCGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG10.19607843137254902No Hit
GGAACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAAATAAAAAC10.19607843137254902No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGCACC10.19607843137254902No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTACTTCAATTAAATAC10.19607843137254902No Hit
GGCACTAGAATGGACCTCTTCTCATCACTACATTATTTCAATTAAATACA10.19607843137254902No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCGTG10.19607843137254902No Hit
AAATACCGTGCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG10.19607843137254902No Hit
GGCACTAGAAAGGACCTCTTCCTCATCACTACAATATTTCAATTAAATAC10.19607843137254902No Hit
GGCACTAGAATGGGACCTCTTCCTCATCACTACATTATTTCAATTAAATA10.19607843137254902No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTAAATTATTTCAATTAAATAC10.19607843137254902No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGTTGCTGGAACC10.19607843137254902No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCCTTGTTTTTAAGTAA10.19607843137254902Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACAATG10.19607843137254902No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATAAAATAC10.19607843137254902No Hit
GGCATAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACAA10.19607843137254902No Hit
GGCACTGAATGGACCTCTTCGTTGATAGCACTGCCTTGGTTTTTAAGTAA10.19607843137254902No Hit
GGCACTATAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC10.19607843137254902No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)