FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054
Total Bases152.7 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC23121.916508538899432No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC21220.113851992409867No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG15414.61100569259962No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC14213.472485768500949No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG474.459203036053131No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC363.415559772296015No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT131.2333965844402277No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG111.0436432637571158No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT80.7590132827324478No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.47438330170777987No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.47438330170777987No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT50.47438330170777987No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.47438330170777987No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.3795066413662239No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.3795066413662239No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.2846299810246679No Hit
AAATACCATTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC30.2846299810246679No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.2846299810246679No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.2846299810246679No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.2846299810246679No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC30.2846299810246679No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.2846299810246679No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT30.2846299810246679No Hit
AAATACCGTTAAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC20.18975332068311196No Hit
AAATACCGTTCAGAGTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT20.18975332068311196No Hit
AAATACCGTTCAGAGCTGCCTTATTTTTAAGTAAGACGTGTAGAACCATG20.18975332068311196No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATAGCTGCTGGAACC20.18975332068311196No Hit
AAATACCGTTCGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.18975332068311196No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA20.18975332068311196No Hit
AAATACCGTTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAAC20.18975332068311196No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCGAATTGCTGCTGGAACC20.18975332068311196No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTACTGGAACC20.18975332068311196No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA20.18975332068311196No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.18975332068311196No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGTTTGCAAATTGCTGCTGGAACC20.18975332068311196No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)