FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2867
Total Bases415.6 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC54719.07917683990234No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC48616.95151726543425No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC47116.428322288106035No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG31310.917335193582142No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC31010.812696198116498No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT883.069410533658877No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG812.825252877572375No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG200.6975933031042902No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT180.6278339727938612No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.38367631670735963No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT110.38367631670735963No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA100.3487966515521451No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.2790373212417161No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTAAATTATTTCAATTAAATAC80.2790373212417161No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.2790373212417161No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.24415765608650158No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.24415765608650158No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.20927799093128704No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.20927799093128704No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.17439832577607256No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.17439832577607256No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.17439832577607256No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.17439832577607256No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.17439832577607256No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA40.13951866062085805No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.13951866062085805No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.13951866062085805No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.13951866062085805No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA40.13951866062085805No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT40.13951866062085805No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.13951866062085805No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT40.13951866062085805No Hit
AAAACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.10463899546564352No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.10463899546564352No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.10463899546564352No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.10463899546564352No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGTTGGAACC30.10463899546564352No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC30.10463899546564352No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCCGCTGGAACC30.10463899546564352No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.10463899546564352No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.10463899546564352No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.10463899546564352No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGGACC30.10463899546564352No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTATATTATTTCAATTAAATAC30.10463899546564352No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.10463899546564352No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT30.10463899546564352No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCCGGAACC30.10463899546564352No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.10463899546564352No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC30.10463899546564352No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.10463899546564352No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT30.10463899546564352No Hit
AAATACCGTTCAGAGCTGCCACCAAGGGCTTGCAAATTGCTGCTGGAACC30.10463899546564352No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)