FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093
Total Bases158.4 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC31628.91125343092406No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC27725.343092406221405No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG14313.083257090576394No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG746.77035681610247No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC373.385178408051235No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG141.2808783165599267No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT111.0064043915827996No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA80.7319304666056725No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT80.7319304666056725No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA70.6404391582799633No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC70.6404391582799633No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.5489478499542544No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.36596523330283626No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.36596523330283626No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA40.36596523330283626No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.36596523330283626No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.36596523330283626No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC30.2744739249771272No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.2744739249771272No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTCGCAAATTGCTGCTGGAACC30.2744739249771272No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.2744739249771272No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT30.2744739249771272No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.2744739249771272No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA30.2744739249771272No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.2744739249771272No Hit
AAAACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.18298261665141813No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAA20.18298261665141813No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC20.18298261665141813No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.18298261665141813No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.18298261665141813No Hit
AAATACCGTTCAGAGCTGCCGCCAGGAGCTTGCAAATTGCTGCTGGAACC20.18298261665141813No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGATGGAACC20.18298261665141813No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC20.18298261665141813No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.18298261665141813No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.18298261665141813No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.18298261665141813No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC20.18298261665141813No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT20.18298261665141813No Hit
GGCACTAGAATGGACCTCTTCTCATCACTACATTATTTCAATTAAATACA20.18298261665141813No Hit
AAATACCGTTCAGACTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.18298261665141813No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)