FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3075
Total Bases445.6 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC89429.073170731707314No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC60419.642276422764226No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC2779.008130081300813No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG2758.94308943089431No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC2708.780487804878048No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG973.1544715447154474No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT220.7154471544715447No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA160.5203252032520325No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG160.5203252032520325No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA120.3902439024390244No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT120.3902439024390244No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA110.35772357723577236No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA100.3252032520325203No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA100.3252032520325203No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA100.3252032520325203No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.2926829268292683No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA90.2926829268292683No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA80.26016260162601623No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA80.26016260162601623No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT80.26016260162601623No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.22764227642276422No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.22764227642276422No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTAAATTATTTCAATTAAATAC70.22764227642276422No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA70.22764227642276422No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT70.22764227642276422No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT70.22764227642276422No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.1951219512195122No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT60.1951219512195122No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.1951219512195122No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.1951219512195122No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.1951219512195122No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.16260162601626016No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC50.16260162601626016No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.16260162601626016No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.13008130081300812No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC40.13008130081300812No Hit
GGCACTAGAATGGACCTCTTCATCATCACTACATTATTTCAATTAAATAC40.13008130081300812No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.13008130081300812No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.13008130081300812No Hit
AAATCCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT40.13008130081300812No Hit
GGCACTAGAATGGACCTCTTCCTCATCCCTACATTATTTCAATTAAATAC40.13008130081300812No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)