FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280
Total Bases185.5 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC36428.4375No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC19014.84375No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC16212.65625No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG13410.46875No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC1027.968749999999999No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG574.453125No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT221.7187500000000002No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG80.625No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA60.46875No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA50.390625No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT50.390625No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT50.390625No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA50.390625No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.3125No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA40.3125No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.3125No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.3125No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.3125No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.3125No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.3125No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.234375No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.234375No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.234375No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTACATTATTTCAATTAAATAC30.234375No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.234375No Hit
AGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC20.15625No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.15625No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCGATTAAATAC20.15625No Hit
AAATACCGTTCGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.15625No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.15625No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCCTGCAAATTGCTGCTGGAACC20.15625No Hit
AAATACCGTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.15625No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTCGCAAATTGCTGCTGGAACC20.15625No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCCGGAACC20.15625No Hit
GGCACTAGAATGGACCTCTTCCTCCTCACTACATTATTTCAATTAAATAC20.15625No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.15625No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.15625No Hit
AAATACCGTTAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.15625No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT20.15625No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT20.15625No Hit
GGCACTAGAATGGACCTCCTCCTCATCACTACATTATTTCAATTAAATAC20.15625No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTAAATTATTTCAATTAAATAC20.15625No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)