FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865
Total Bases125.4 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC18221.040462427745666No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC15918.381502890173408No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC15317.6878612716763No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG12714.682080924855493No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG455.202312138728324No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC141.6184971098265895No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG80.9248554913294799No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT60.6936416184971098No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT60.6936416184971098No Hit
AAATACCTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.46242774566473993No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.46242774566473993No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.46242774566473993No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.46242774566473993No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT40.46242774566473993No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.46242774566473993No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.46242774566473993No Hit
AAATACCGTTCAGAGCGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.3468208092485549No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.3468208092485549No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.3468208092485549No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.3468208092485549No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGACCC20.23121387283236997No Hit
AAATACCGTTCAGACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.23121387283236997No Hit
AAAACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.23121387283236997No Hit
AAATACCGTTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAAC20.23121387283236997No Hit
GGCACTAGAATGGACCTCTTCCTCATCATTACATTATTTCAATTAAATAC20.23121387283236997No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAGCC20.23121387283236997No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGACCC20.23121387283236997No Hit
AAATACCGTTAAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.11560693641618498No Hit
AAATACCGTTCAGAGTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.11560693641618498No Hit
GGCACTAGAATGGACCCCTTCCTCATCACTACATTATTTCAATTAAATAC10.11560693641618498No Hit
GACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACAA10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCTTATTTTTAAGTAAGACGTGTAGAACCATG10.11560693641618498No Hit
AATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCTG10.11560693641618498No Hit
AAAACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.11560693641618498No Hit
AAATACTGTTCAGAGCTGCCACCAAGAGCTTGCTAATTGCTGCTGGAACC10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAATAAGACGTGTAGAACCATGT10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTCCTGGAACC10.11560693641618498No Hit
GGCACAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACAA10.11560693641618498No Hit
GGCATGAGTGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACAA10.11560693641618498No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAACTAAATAC10.11560693641618498No Hit
GGCACAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATACA10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCACAGAAACACACACACAAAGACGTGTAGAA10.11560693641618498No Hit
AATACCGTTCAGACTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCTG10.11560693641618498No Hit
AAAACCGTCAAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGTCG10.11560693641618498No Hit
AAATACGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCTG10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCCTGCAAATTGCTGCTGGAACC10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCCACAGAAACACACACACAAAGACGTGTAGA10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAATAAGACGTGTAGAACCATGT10.11560693641618498No Hit
GGCACTGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACAA10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGAAACC10.11560693641618498No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.11560693641618498No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.11560693641618498No Hit
AAATACCGTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.11560693641618498No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCACTAGGAGCTTGCAAATTGCTGCTGGAACC10.11560693641618498No Hit
AAATACCGATCAGAGCTGCCACCACGAGCTTGCAAATAGCTGCTCGAACA10.11560693641618498No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCAGGAACC10.11560693641618498No Hit
AAATACCGTTCAGAGTTGCCACCAAGAGCTTGCAAATTACTGCTGGAACC10.11560693641618498No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAGATTATTTCAATTAAATAC10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCATCAGGAGCTTGCAAATTGCTGCTGGAACC10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCAACAAGAGCTAGCAAATTGAAGCTCGAACC10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTACTGCTGGAACC10.11560693641618498No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.11560693641618498No Hit
GGACTAGAATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATACA10.11560693641618498No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTATATAC10.11560693641618498No Hit
AAATACCGTTCAGAGCTCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.11560693641618498No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTAGTTCAATTAAATAC10.11560693641618498No Hit
GGACTAGAATCGACCTCTACCTCAACACTACATTAATTCAAATAAATACA10.11560693641618498No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAAAAC10.11560693641618498No Hit
GGCACTAGAATGGACCTCTTCCTCATCACGACATTATTTCAATTAAATAC10.11560693641618498No Hit
AAATACCGTTCAGAGCTGTCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGCACCTGGGAGGGCACAGCACT10.11560693641618498No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.11560693641618498No Hit
AAATACCGTTCAGGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.11560693641618498No Hit
AAATACCGTTCAGGGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCACCAAGCGCTTGCAAATTGCTGCTGGAACC10.11560693641618498No Hit
GGCACTAGATGGACCTCTCCCTCATCACTACATTATTTCAATTAAATACA10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTCGCAAATTGCTGCTGGAACC10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGTTGCTGGAACC10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTTTAGAACCATG10.11560693641618498No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTCCATGTACTCTGCG10.11560693641618498Clontech Universal Primer Mix Long (95% over 22bp)
GGCACTAGAATGGACCTCTTCCTCATCACTACAATATTTCAATTAAATAA10.11560693641618498No Hit
GGCACTAGAATGGACCCTTCCTCATCACTACATTATTTCAATTAAATACA10.11560693641618498No Hit
AAAAGCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.11560693641618498No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTACATTATTTCAATTAAATAC10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCACCAAGAACTTGCAAATTGCTGCTGGAACC10.11560693641618498No Hit
GGTACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAACAC10.11560693641618498No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.11560693641618498No Hit
AAAAACCGTTCAGAGCTGCCACAAGGAGCTTGCAAATTTCTGCTGGAACC10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAAACATG10.11560693641618498No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC10.11560693641618498No Hit
AAATACCGTTCAGAGCTGACACCAAGAGCTTGCAAATTGCTGCTGGAACC10.11560693641618498No Hit
AAATACCGTTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT10.11560693641618498No Hit
AAATCCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.11560693641618498No Hit
GGCACTAGAATGGACCTATTCCTCATCACTACATTATTTCAATTAAATAC10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATCGCTGCTGGAACC10.11560693641618498No Hit
AAATACCTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGAAAATTGCTGCTGGAACC10.11560693641618498No Hit
AAACACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC10.11560693641618498No Hit
AAATACCGTTCAGTGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGATTGCAAATTGCTGCTGGAACC10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATC10.11560693641618498No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTCCAATTAAATAC10.11560693641618498No Hit
AAATACCGTTCAGATCTGCAACCAGGAGCTTGCAAATTGCTGCTGGAACC10.11560693641618498No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAGCT10.11560693641618498No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATAGCTGCTGGAACC10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCTTTTTTTAAGTAAGACGTGTAGAACCATGT10.11560693641618498No Hit
AAATACCGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.11560693641618498No Hit
AAATACTGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC10.11560693641618498No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACGTTATTTCAATTAAATAC10.11560693641618498No Hit
AAATACCGTTCAGAACTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.11560693641618498No Hit
AATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCTG10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGGACC10.11560693641618498No Hit
AAATACCGTTCAAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCTTGTTTAAGTAAGACGTGTAGAACCATGTC10.11560693641618498No Hit
ACATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC10.11560693641618498No Hit
AGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTTCTGCTGGACCC10.11560693641618498No Hit
GGCACTAGAATGGACCTCTTCGTTGATACCACTGCCTTGTTTTTAAGTAA10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTCCAAATTGCTGCTGGAACC10.11560693641618498No Hit
AAATACCGTTCAGGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.11560693641618498No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC10.11560693641618498No Hit
GGCACTAGAATGGACCTCTTCCTCATAACTACATTATTTCAATTAAATAC10.11560693641618498No Hit
GGCACTAGAATGGACCTCTTCCTTATCACTACATTATTTCAATTAAATAC10.11560693641618498No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT10.11560693641618498No Hit
GGCATAGATGGACCTCGTCCTCATCACTAAATTATTTCAATTAAATACAA10.11560693641618498No Hit
GGCACTAGAATGGACTTCTTCCTCATCACTACATTATTTCAATTAAATAC10.11560693641618498No Hit
AAATACAGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.11560693641618498No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.11560693641618498No Hit
AAATACCGTTCAGACTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCACCAGGATCTTGAAAATTGCTGCTGGAACC10.11560693641618498No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCCTTGTTTTTAAGTAA10.11560693641618498Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.11560693641618498No Hit
AAATACATATCAGACATGCCACCAGGAGCTTTCAAAATGCGGCTTGACAA10.11560693641618498No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGTAACC10.11560693641618498No Hit

[FAIL]Adapter Content

Adapter graph