FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086
Total Bases157.4 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC27425.23020257826888No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC16515.193370165745856No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG13412.338858195211786No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC12711.694290976058932No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC1029.392265193370166No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG504.6040515653775325No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT312.85451197053407No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG70.6445672191528545No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.5524861878453038No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.5524861878453038No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.3683241252302026No Hit
AAATACCGTTAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.3683241252302026No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC30.2762430939226519No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.2762430939226519No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.2762430939226519No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.2762430939226519No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.2762430939226519No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.2762430939226519No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT30.2762430939226519No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT30.2762430939226519No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT30.2762430939226519No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.1841620626151013No Hit
AAATACCGTTCAGAGTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT20.1841620626151013No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.1841620626151013No Hit
GGCACTAAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.1841620626151013No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.1841620626151013No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAGT20.1841620626151013No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA20.1841620626151013No Hit
AAATACCGTTCGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT20.1841620626151013No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGATGCTGGAACC20.1841620626151013No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACACTATTTCAATTAAATAC20.1841620626151013No Hit
AAATACCTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.1841620626151013No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCCGCTGGAACC20.1841620626151013No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTAAATTATTTCAATTAAATAC20.1841620626151013No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)