FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912
Total Bases132.1 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC16217.763157894736842No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC14515.899122807017543No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG14015.350877192982457No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC12713.925438596491228No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC808.771929824561402No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG596.469298245614036No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT232.5219298245614032No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG60.6578947368421052No Hit
GGCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA50.5482456140350876No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.43859649122807015No Hit
AATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT40.43859649122807015No Hit
GGCACTGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.3289473684210526No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA30.3289473684210526No Hit
GCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA30.3289473684210526No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT30.3289473684210526No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.21929824561403508No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTTCATTATTTCAATTAAATAC20.21929824561403508No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTACATTATTTCAATTAAATAC20.21929824561403508No Hit
AAATACCGTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.21929824561403508No Hit
GGCACTAGAATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACA20.21929824561403508No Hit
GGCACTAGAATGGACCTCTCCTCATCACTAAATTATTTCAATTAAATACA20.21929824561403508No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAGATTGCTGCTGGAACC20.21929824561403508No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA20.21929824561403508No Hit
GGCATTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC20.21929824561403508No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAAACATG20.21929824561403508No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.21929824561403508No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC20.21929824561403508No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.21929824561403508No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA20.21929824561403508No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT20.21929824561403508No Hit
AAATACCTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT20.21929824561403508No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTACATTATTTCAATTAAATAC20.21929824561403508No Hit
AATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACTG20.21929824561403508No Hit
AATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.21929824561403508No Hit
AAATACGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT20.21929824561403508No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCAGTAGCTTGCAAATAGCTGCTGGAAAC10.10964912280701754No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAAATATTTCAATTAAAAAC10.10964912280701754No Hit
AATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.10964912280701754No Hit
AAAACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTTCAAATTGCTGCTGGAACC10.10964912280701754No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA10.10964912280701754No Hit
GGCACTAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACAA10.10964912280701754No Hit
AAATACCGTCCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.10964912280701754No Hit
AAATCCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.10964912280701754No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAACACA10.10964912280701754No Hit
AAATACCGTTCAGAACTGCCTTGTTATTAAGTAAGACGTGTAGAACCATG10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGAAAATTGCTTCTGGAAAC10.10964912280701754No Hit
AAATACCTTTCAGAGCTGCCACCAGGAGCTTGAAAATTGCTGCTGGAACC10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCAGGAACC10.10964912280701754No Hit
GGCACTAGAATGGCCTCTTCCTCATCACTACATTACTTCAATTAAATACA10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCCTGCAAATTGCTGCTGGAACC10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCTTGTATTTAAGTAAGACGTGTAGAACCATG10.10964912280701754No Hit
AATCCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCTG10.10964912280701754No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTTCCATGTACTCTGC10.10964912280701754Clontech Universal Primer Mix Long (95% over 23bp)
GCACTAGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACAA10.10964912280701754No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.10964912280701754No Hit
GGCATAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCACCAGGAGCTTGCAAATTGCTGCTGGAACCT10.10964912280701754No Hit
GGCACTAGAATGGACCTCTTCCTCATAACTAAATTATTTCAATTAAATAC10.10964912280701754No Hit
GGCACAGAATGGACCTCGTCCTCATCACTACATTATTTCAATTAAATACA10.10964912280701754No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAACAC10.10964912280701754No Hit
AAATACCGTTCAGAGCTTCCACCAAGAGCTTGCAAATTGCTGCTGGAACC10.10964912280701754No Hit
GGCACTAGAATGGACCTCATCCTCATCACTACATTATTTCAATTAAATAC10.10964912280701754No Hit
AAAAACGTTCAGAGCTGCCACCAAGAGCTGGCAAATTGCTGCTGGAACCT10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGATGCTGGAACC10.10964912280701754No Hit
AAATACCGTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCGAATTGCTGCTGGAACC10.10964912280701754No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATGCA10.10964912280701754No Hit
AATACCTTTCAGAGCTGCCACCGGGAGCTTGCAAATTTCTGCTGTAACCT10.10964912280701754No Hit
GGCACTAGAATGGACCTCTTCCTCATCAATACATTATTTCAATTAAATAC10.10964912280701754No Hit
GAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.10964912280701754No Hit
AAATACCGTTCAGAGCAGCCACCAAGAGCTAGCAAATTGCTGCTCGAACC10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCCCCAAGAGCTTGCAAATTGCTGCTGGAACC10.10964912280701754No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA10.10964912280701754No Hit
GGCACTAGAATGGCCCTCTCCCTCATCACTACATTATTTCAATTAAATAC10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTCGAACC10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCACGAGCTTCCAAATAGCTGCTGGAACC10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTCCTGCTGGAACC10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCAAGTTCTGTGTCTTGATAACCTTTCCTTCC10.10964912280701754No Hit
AAAACCGTTCAGAGCTGCCACCAAGAGCTTGTAAATTGCTGCTGGAACCT10.10964912280701754No Hit
AAATACCGTTCAGCACCGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG10.10964912280701754No Hit
GGACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.10964912280701754No Hit
AAAAAAAGTTAAGACCTGACCCCAAGAGCTTTAATATTTCTGATAGAAAC10.10964912280701754No Hit
GAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACC10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCTAGTTTTTAAGTAAGACGTGTAGAACCAAG10.10964912280701754No Hit
AAATACCGTTAAGAGCTGCCACCAGTAGCCTCCAAATTGATGCTGGAACC10.10964912280701754No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA10.10964912280701754No Hit
GGACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACAA10.10964912280701754No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT10.10964912280701754No Hit
AAATACCGTTCAGAACTACCTTGTTTTTAAGTAAGACGTGTAGAACCATG10.10964912280701754No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.10964912280701754No Hit
GGCACTAGAATGGACCTCTGCGTTGATACCACTGCTACCCACAGAAACAC10.10964912280701754Clontech Universal Primer Mix Long (95% over 22bp)
GGACTAGAATGCACCTCTTCATCATCAATACATTATTACAATTAAATACA10.10964912280701754No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATTT10.10964912280701754No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA10.10964912280701754No Hit
AGGCACTAGAATGGACCTCTTCGTTGATACCACTGCTTCCATGTACTCTG10.10964912280701754Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAATACCGTTCAGAGCTGCCACCGAGAGCTTGCAAATTGCTGCTGGAACC10.10964912280701754No Hit
AAATACAGTTCAGAGCTGCCTTGTTTTTAAGTAATAAGTGTAGAACCAAG10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGATGGAACC10.10964912280701754No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCGAATTGCTGCTGGAACC10.10964912280701754No Hit
AAATACCGTTCGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACTGCC10.10964912280701754No Hit
AAAAACCGTTCAGAGCAGCCACCAAGAGCTTGCAAATTGCTGCTCGAACC10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT10.10964912280701754No Hit
AAATACCGTTCAGCGCTGACACCAGGAGCTTGCAAATTGCTGATGGACCC10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTAGAACC10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGAAAATTGCTGCTGGAACC10.10964912280701754No Hit
AAATACCGTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.10964912280701754No Hit
AAATACCGTTAAGAGCTGCCACCAAGAGCTAGCAAATTGCTGCTGGAACC10.10964912280701754No Hit
GGCACTGATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACAA10.10964912280701754No Hit
GGCACTAGAATGGACCTCTTCATCATCACTACATTATTTCAATTAAATAC10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAGATTGCTGCTGGAACC10.10964912280701754No Hit
GGCACAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCAATAGCTTGCAAATTGCTGCTGGAACC10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCTTTTTTTAAGTAAGACGTGTAGAACCATGT10.10964912280701754No Hit
AAATACGTTCAAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCTG10.10964912280701754No Hit
AAATACGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACTG10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTAGCAAAATTCTGCTGGAACC10.10964912280701754No Hit
GGCACTAGAATGGACCTCTGCCTCATCACTACATTATTTCAATTAAATAC10.10964912280701754No Hit
AATACCGTTCAGAGCTGCCCCCCGGAGCTTGCAAATTGCTCCTGGAAACT10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGAAAATATCTGCTGGAACC10.10964912280701754No Hit
AAATCCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGAAAATTGATGCTGGAACC10.10964912280701754No Hit
AAATACAGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG10.10964912280701754No Hit
AGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC10.10964912280701754No Hit
GGCATGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACAA10.10964912280701754No Hit
GGCACTGATGGACTCTTCCTCATCACTACATTATTTCAATTAAATACAAT10.10964912280701754No Hit
GGCACTAGAATGGACCTCTTCCCCATCACTAAATTATTTCAATTAAATAC10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACG10.10964912280701754No Hit
GGCACTAGAATGGACCTCCTCCTCATCACTACATTATTTCAATTAAATAC10.10964912280701754No Hit
AAATACCGTTCAGCGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.10964912280701754No Hit
AAACACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC10.10964912280701754No Hit
AAATACGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCTGCTGGAACCT10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCGGGAGCTTGCAAATTGCTGCTGGAACC10.10964912280701754No Hit
GGCACTAGAATGGACCTCTTCTCATCACTACATTATTTCAATTAAATACA10.10964912280701754No Hit
AAATACCGTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACTG10.10964912280701754No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAATTAC10.10964912280701754No Hit
AAATACCGTTCAGAGCTGCCACCAAGAGCTTGCAAATTGCAGCTGGAACC10.10964912280701754No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTATATTATTTCAATTAAATAC10.10964912280701754No Hit

[FAIL]Adapter Content

Adapter graph