FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3626
Total Bases525.5 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATAC130235.907335907335906No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACC93225.703254274682845No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG43511.996690568119138No Hit
AAATACCGTTCAGAACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG1403.861003861003861No Hit
GGCACTAGAATGGACCTCTTCCTCATCACTACATTATTTCAATTAAATAC1052.8957528957528957No Hit
AATACCGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT240.6618863761720905No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCACAGCACT220.6067291781577496No Hit
GGCACTAGATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA220.6067291781577496No Hit
AAATACCGTTCAGCACTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATG220.6067291781577496No Hit
GCACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA150.4136789851075565No Hit
GGCACTAAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.3861003861003861No Hit
GGCATAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA140.3861003861003861No Hit
GGCACTAGAATGGACTCTTCCTCATCACTAAATTATTTCAATTAAATACA130.35852178709321564No Hit
AATACCGTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCATGT130.35852178709321564No Hit
GGCACTAGAATGGACCTCTTCGTTGATAGCACTGCCTTGTTTTTAAGTAA130.35852178709321564No Hit
GGCACAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA120.3309431880860452No Hit
AAATACGTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT110.3033645890788748No Hit
GGCACTAGAATGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.3033645890788748No Hit
GGCCTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA110.3033645890788748No Hit
AAATACCGTTAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT100.27578599007170435No Hit
AAATACCGTTCAGAGCTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT100.27578599007170435No Hit
GGACTAGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA90.2482073910645339No Hit
AAATACCGTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT70.19305019305019305No Hit
GGCACTAGAATGGACCTCTCCCTCATCACTAAATTATTTCAATTAAATAC60.1654715940430226No Hit
AAATACCGTTTCAGAGCTGCCTTGTTTTTAAGTAAGACGTGTAGAACCAT60.1654715940430226No Hit
GGCACTGAATGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA60.1654715940430226No Hit
GGCACTAGAATGGACCTCTTTCTCATCACTAAATTATTTCAATTAAATAC50.13789299503585217No Hit
AAATACCGTTCGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAACCT50.13789299503585217No Hit
AAATACCGTTCAGAGCTGCCACCAGGAGCTCGCAAATTGCTGCTGGAACC50.13789299503585217No Hit
GGCACTAGAATGGCCCTCTTCCTCATCACTAAATTATTTCAATTAAATAC50.13789299503585217No Hit
AAATACCGTTCAGAACTGCCTTGTTTTAAGTAAGACGTGTAGAACCATGT50.13789299503585217No Hit
GGCACTAGAATGACCTCTTCCTCATCACTACATTATTTCAATTAAATACA40.11031439602868175No Hit
GGCACTAGAAGGACCTCTTCCTCATCACTAAATTATTTCAATTAAATACA40.11031439602868175No Hit
GGCACTAGAATGGACCTCTTCGTCATCACTAAATTATTTCAATTAAATAC40.11031439602868175No Hit
AAATACCGTTTCAGAGCTGCCACCAGGAGCTTGCAAATTGCTGCTGGAAC40.11031439602868175No Hit
GGCACTAGAATGGACCTCTCCTCATCACTAAATTATTTCAATTAAATACA40.11031439602868175No Hit
GGCACTAGAATGGACCCTTCCTCATCACTAAATTATTTCAATTAAATACA40.11031439602868175No Hit
AAATACCGTTCAGAGCTGCCAGCTTGGGGTACCTGGGAGGGCATAGCACT40.11031439602868175No Hit
GGCACTAGAATGGACCTCTTCTCATCACTAAATTATTTCAATTAAATACA40.11031439602868175No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)