FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363
Total Bases52.6 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG11130.57851239669421No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT6517.90633608815427No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC5916.2534435261708No Hit
ACTTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTC287.7134986225895315No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC287.7134986225895315No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG82.203856749311295No Hit
ACTTAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT30.8264462809917356No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC30.8264462809917356No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC30.8264462809917356No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC30.8264462809917356No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC30.8264462809917356No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTCGTGAGACCAAGTCTCT20.5509641873278237No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT20.5509641873278237No Hit
ACTTAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT20.5509641873278237No Hit
ACTTAAAAACAAGGCAGTGGTATCAACGCAGAGGTCCATTCTAGTGCCTT20.5509641873278237Clontech Universal Primer Mix Long (95% over 22bp)
CTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.27548209366391185No Hit
ACTTAAAAAAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.27548209366391185No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTGATGGTGCTACAGTTTCTGCCT10.27548209366391185No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC10.27548209366391185No Hit
ACTTAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.27548209366391185No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTATCTGCCT10.27548209366391185No Hit
ACTTAAAAACAAGGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCC10.27548209366391185No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGATACAGTTTCTGCCT10.27548209366391185No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGTTACAGTTTCTGCCT10.27548209366391185No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACGGTTTCTGCCT10.27548209366391185No Hit
TGTTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.27548209366391185No Hit
TGTGTGTGTGTTTCGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.27548209366391185No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGTCT10.27548209366391185No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTTCCT10.27548209366391185No Hit
ATTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.27548209366391185No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTTTTCGTGAGACCAAGTCTCTGC10.27548209366391185No Hit
ACTTAAAAACAAGGCAGTGCTAGTGCCTAATGGTGCTACAGTTTCTGCCT10.27548209366391185No Hit
TGTGTGTGTGTTTTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.27548209366391185No Hit
ACTTAAAAACAAGGCAGTTCTAATGCCTAATGGTGCTACAGTTTCTGCCT10.27548209366391185No Hit
ACTTAAAAACAAGGCAGCTCTTAACGGTATTTTTCGTGAGACCAAGTCTC10.27548209366391185No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCCCCCAGGTACCCCAAGCTGGCAG10.27548209366391185No Hit
ACTTAAAAACAAGGCAGTTCTAATGCTAATGGTGCTACAGTTTCTGCCTC10.27548209366391185No Hit
TGTGTGTGTGTTTTTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG10.27548209366391185No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.27548209366391185No Hit
ACTTAAAAACAAGGCAGTTCGAACGGTATTTTTCGTGAGACCAAGTCTCT10.27548209366391185No Hit
ACTTAAAAACAAGGCAGTGATAATGCCTAATGGTGCTACAGTTTCTGCCT10.27548209366391185No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCGAATGGTGCTACAGTTTCTGCCT10.27548209366391185No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTGCAGTTTCTGCCT10.27548209366391185No Hit
ACTTAAAAAGGCAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCTTC10.27548209366391185No Hit
ACTTAAAAACAAGGAGTGCTAATGCTAATGGTGCTACAGTTTCTGCCTCT10.27548209366391185No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTTTTTTCGTGAGACCAAGTCTCT10.27548209366391185No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGTAGGGTAAAG10.27548209366391185No Hit
TCTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG10.27548209366391185No Hit
ACTTAAAAACAAGGCAGGTCTGATCGGTATTTTTCGTCAGTCACTGGCTC10.27548209366391185No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTCGTGAGACCAAGTCTCT10.27548209366391185No Hit
ACTTAAAAACAAGGCGGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC10.27548209366391185No Hit
ACTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT10.27548209366391185No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAATGTTTGGACAGAAGGGTAAAG10.27548209366391185No Hit
TGTGTGTGTGTTTCAGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG10.27548209366391185No Hit
ACTAAAAACAAGGCAGTGCTAATGCTAATGGTGCTACAGATTCTGCCTCT10.27548209366391185No Hit
TGTGTGTTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC10.27548209366391185No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)