FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008151501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008151501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2985
Total Bases432.8 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG120840.46901172529313No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT92030.82077051926298No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTC32410.854271356783919No Hit
ACTTAAAAACAAGGCAGTTCTGAACGGTATTTTTCGTGAGACCAAGTCTC963.2160804020100504No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTACCCCAAGCTGGCAG662.21105527638191No Hit
ACTTAAAAACAAGGCAGTGCTGAACGGTATTTTTCGTGAGACCAAGTCTC240.8040201005025126No Hit
ACTTAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC190.6365159128978224No Hit
GTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC70.23450586264656617No Hit
TGTGTGTGTGTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC70.23450586264656617No Hit
TGTGTGTTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC60.20100502512562815No Hit
ACTTAAAAACAAGGCAGCTCTGAACGGTATTTTCGTGAGACCAAGTCTCT60.20100502512562815No Hit
ACTTAAAAACAAGGCAGTGCTATCAACGAAGAGGTCCATTCTAGTGCCTT50.16750418760469013No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAGGGGTAAAG50.16750418760469013No Hit
TGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGCT50.16750418760469013No Hit
ACTTAAAAAAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC50.16750418760469013No Hit
ACTTAAAAACAAGGCAGTGCTATGCCCTCCCAGGTACCCCAAGCTGGCAG50.16750418760469013No Hit
ACTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC50.16750418760469013No Hit
ACTTAAAAACAAGGCAGTGCTGTGCCCTCCCAGGTATAGGGGCACTCAGA40.1340033500837521No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTGAAG40.1340033500837521No Hit
ACTTAAAAACAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC40.1340033500837521No Hit
TGTGGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC40.1340033500837521No Hit
ACTTAAAAACAAGGCAGTGCTAATGCCTAATGGTGCTACAGTTTCCGCCT40.1340033500837521No Hit
ACTAAAAACAAGGCAGCTCTGAACGGTATTTTTCGTGAGACCAAGTCTCT40.1340033500837521No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGGCAGAAGGGTAAAG40.1340033500837521No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAGG30.10050251256281408No Hit
ACTTAAAAACAAGCAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCTC30.10050251256281408No Hit
ACTTAAAAACAAGGCAGTGTTAATGCCTAATGGTGCTACAGTTTCTGCCT30.10050251256281408No Hit
TGTGTGTGTGTTTCTTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC30.10050251256281408No Hit
TGTGTGTGTGTTTCTGTGGGTTTCCTTAAGGTTTGGACAGAAGGGTAAAG30.10050251256281408No Hit
TGTGTGGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC30.10050251256281408No Hit
ACTTAAAAACAAGGCAGCTCTGACGGTATTTTTCGTGAGACCAAGTCTCT30.10050251256281408No Hit
TGTGTGTGTGTTTCTGGGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAG30.10050251256281408No Hit
TGTTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACAGAAGGGTAAAGC30.10050251256281408No Hit
TGTGTGTGTGTTTCTGTGGGTTTCTTTAAGGTTTGGACGGAAGGGTAAAG30.10050251256281408No Hit
ACTTAAAAACAAGGTAGTGCTAATGCCTAATGGTGCTACAGTTTCTGCCT30.10050251256281408No Hit
ACTTAAAAACAAGGCAGCTCTAACGGTATTTTTCGTGAGACCAAGTCTCT30.10050251256281408No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)